Dorota
Łoboda
and
Magdalena
Rowińska-Żyrek
*
Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland. E-mail: magdalena.rowinska-zyrek@chem.uni.wroc.pl
First published on 4th July 2017
The aim of this work is to understand the interactions of Zn(II) with Pra1, a zincophore from Candida albicans, one of the most common causes of serious fungal infections in humans. Pra1 is a 299 amino acid protein, secreted from the fungus to specifically bind Zn(II) and deliver it to a transmembrane zinc transporter, Zrt1. We take the first step towards understanding the bioinorganic chemistry of this process, by pointing out the Zn(II) binding sites in Pra1 and understanding the thermodynamics of such interactions. Our approach involves working on model systems (unstructured parts of proteins) in order to identify those regions in Pra1, to which zinc binds with the highest affinity. Mass spectrometry shows the stoichiometry of Zn(II)–peptide complex formation and potentiometric studies give us the partial and overall stability constants for all the formed zinc complexes. NMR clarifies binding sites in the case of doubts. A detailed comparison of these results shows that the C-terminal region of Pra1 binds Zn(II) with the highest affinity, indicating that this region of the zincophore is responsible for the binding of zinc. Such knowledge is an input to the basic bioinorganic chemistry of zinc; it allows us to understand the inorganic biochemistry of zincophores, and it might be a stepping stone towards finding new, fungus specific treatments based on parts of zincophores coupled with antifungal drugs.
One of the biggest obstacles in finding new fungus-specific therapeutics comes from the fact that fungi share essential metabolic pathways and core cellular machineries with humans, much more than disease-causing bacteria.5 In order to design a highly specific antifungal drug, it is crucial to understand and aim at differences in the human and fungal metabolism.6
Although pathogen-selective targets are scarce, there is at least one significant difference between fungal and mammalian cells: the zinc transport system based on zincophores – present in fungi, absent in humans. Zinc is an essential metal for a plethora of cellular events and therefore is an indispensable micronutrient for almost all living organisms. 9% of fungal proteomes consist of zinc-binding proteins;7,8 a quarter of these proteins are involved in transcriptional regulation, e.g. being zinc finger transcription factors (116 different ones in C. albicans and 311 in A. fumigatus) or regulators of several biological processes, such as amino acid metabolism, nitrogen utilization or cell division.9
Numerous zinc-binding proteins are involved in fungal virulence. Superoxide dismutases (SODs) are the central enzymes in fungi associated with the detoxification of ROS generated by host cells during host–pathogen interactions.10 For this reason, specific SODs from pathogenic fungi are assumed to be virulence determinants. Also the zinc-binding metalloproteases have been shown to be involved in pathogen invasion.11 Examples of such metalloproteases are ADAM metalloproteinases or deuterolysin (with two zinc-binding histidines and a catalytic glutamate in its catalytic centre).12
Beyond any doubt, Zn(II) is crucial for both virulence and survival of fungal pathogens in humans. The task of the uptake of this metal is far from being trivial, with the total host tissue zinc concentration being in the range of 11–23 μM13,14 and the concentration of free Zn(II), not bound to the proteins of the host, is supposed to be as low as 0.1–1 nM.15 The host organism does not make the zinc uptake easier. In fact, the infected host seems to take advantage of the essentiality of this nutrient for fungi and to hamper the pathogen growth, mammals reduce the levels of free zinc (the host restriction of microbial access to certain key nutrients is a process termed nutritional immunity).16,17 This Zn(II) availability restriction is achieved by lowering the availability of free zinc via the activity of the host zinc transporters or the expression of zinc-binding proteins, such as calprotectin, a member of the S100 family of metal-binding proteins, produced by neutrophils in order to reduce the overgrowth of fungi such as Aspergillus fumigatus and C. albicans.18–20
Pathogenic fungi have adapted to these Zn(II)-limiting conditions imposed by the host by developing additional mechanisms to sequester zinc from host cells and tissues – they secrete specific zinc selective chelators, which bind the crucial metal ion and deliver it to the specific fungal membrane transporters. This process is analogous to the well-known iron chelation by secreted siderophores – low molecular weight iron carriers synthesised by bacteria or fungi and secreted into the environment of the host, where they bind ferric ions with high affinity and selectivity. The delivery of iron-loaded siderophores back to the microorganism occurs via specific membrane receptors and transport proteins. An analogous process occurs for zincophores, although the dimensions of the secreted protein, the chemical nature of the process and the mechanism of metal binding are completely different from the ones observed for siderophores.21
The protein based zincophore is a novelty in the scientific world. It was suggested for Pra1, a small, 299 amino acid, secreted zinc binding protein, which can take up this metal from the environment and re-associate with the fungus via a co-expressed, genetically-linked membrane transporter, Zrt1. Recently, Citiulo et al.22 elucidated the mechanism of C. albicans zinc acquisition from host cells: (i) after the host cell invasion Pra1 (pH-regulated antigen 1, the previously mentioned zincophore) is expressed due to the alkaline pH and the low amount of soluble zinc of the intracellular environment;23,24 (ii) the protein is secreted and released from the fungal cell surface, predominantly in the hyphal form; it is required for hyphal extension, but does not directly cause damage of the host; (iii) it binds host cellular zinc and (iv) returns to the fungal cell via a physical interaction with Zrt1, a membrane transporter, to deliver the bound metal ion (Fig. 1).22
Fig. 1 Schematic model of C. albicans zinc scavenging from host cells. After invasion of the host cell, Pra1 is expressed and secreted. It binds zinc, either in the form of free Zn(II) (extremely sparse in the cellular pool) or from zinc-binding proteins of the host reassociation with the C. albicans cell surface and Zn(II) transportation into the cell occurs via a Pra1–Zrt1 interaction.22 |
The PRA1 and ZRT1 genes share their upstream intergenic regions, are transcriptionally co-regulated and exhibit similar expression patterns during the invasion of oral epithelial cells. Hube's group showed that the deletion of the PRA1 gene prevented the utilisation of host zinc and damage of host cells in the absence of exogenous zinc, showing a crucial role of this protein for pathogenicity.22,25
The zinc transport system seems to be very well conserved also in other fungi. Another recent study reported that two genes from A. fumigatus, zrfC and aspF2, share their upstream intergenic regions, are co-regulated and required for growth under zinc limiting conditions,26,27e.g., in the presence of the Zn(II)-chelating protein calprotectin.28
What is most interesting is the fact that Pra1 from C. albicans and AspF2 from A. fumigatus have not only similar properties, but also share 43% of identity upon sequence alignment (Fig. S1†). The same applies to the transmembrane transporter proteins, Zrt1 from C. albicans and ZrfC from A. fumigatus – 48% sequence identity was observed. The percentage of conserved residues in the two Zn(II) transporting proteins is observed in most other fungi.22,27
The aim of this work is to understand the interactions of Zn(II) with Pra1, the C. albicans zincophore. We aimed to investigate bioinorganic chemistry of this process, point out the Zn(II) binding sites in Pra1 and understand the thermodynamics of the binding. We use a peptide-based approach, choosing unstructured regions which are most likely to bind zinc and measuring their precise affinity towards zinc ions.
The most common zinc binding sites are quite well described in the literature.29 The principal ligands that coordinate zinc ions are usually a combination of His, Cys and acidic residues, water molecules and, more rarely, Tyr, Asn, Ser and Thr. The ligand composition and geometry suggest six natural classes: (i) class I, with a ligand group which consists of at least three histidine residues, which share an elongated zinc binding motif EXXXXGXX; (ii) class II, which consists of three His, two of which are arranged in a HXH motif, with the third one being in a more distant position; (iii) class III, being a combination of His and Cys residues; (iv) class IV, in which two histidines, one acidic residue and one water molecule are bound to the zinc ion; (v) class V, predominantly acidic ligands; and (vi) other ligand compositions.22
As far as the sequence of Pra1 is considered, several zinc binding sites can be found when applying the rules described above (highlighted in red in Fig. 2). Another important issue is the structure of the protein. One of our experimental approaches is based on working with model systems – peptides, which are fragments of Pra1. This approach would not be particularly useful if Pra1 had a pre-defined structure. No crystal or solution structure of Pra1 is available; the structure predicted by Phyre2 (a remote homology recognition technique, able to regularly generate reliable protein models)30 shows that especially the C-terminal part of the protein is highly unstructured (Fig. S2†) and therefore appropriate for our peptide-based approach.
The protein parts that are studied have carefully been chosen, based on two criteria: (i) being in an unstructured site and (ii) containing the predicted zinc binding sites. To further confirm that the peptides we have chosen are the ones which are most likely to bind Zn(II), we aligned the sequence of Pra1 from C. albicans with the sequence of Aspf2 (also important for growth under zinc limitation) from A. fumigatus (their sequences share 43% of identity) (Fig. S1†).
The chosen Pra1 fragments include Ac-AEHARDHT-NH2 (residues 66–73), Ac-LHRFWHLK-NH2 (residues 177–184), Ac-IEHY-NH2 (residues 191–194), Ac-SHQHT-NH2 (residues 271–275), and Ac-SHCHTHADGEVHC (residues 287–299). In addition, a longer fragment, which comprised two of the fragments listed above was studied: Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC (residues 271–299).
The HYPERQUAD 2006 program was used for the stability constant calculations.32 Standard deviations were computed by using HYPERQUAD 2006 and referenced to random errors only. The constants for hydrolytic Zn(II) species were used in these calculations.33 The speciation and competition diagrams were computed using the HYSS program.34
In all measured mass spectra, signals corresponding to sodium and potassium adducts of the ligands are observed. In the case of Ac-IEHY-NH2, a potassium adduct is visible (m/z = 640.3, z = 1+). The enlarged signal, which corresponds to the zinc complex, also shows small signals which probably are the result of adding one proton to the peptide during ionization (Fig. S3†). In the Zn(II)-Ac-AEHARDH-NH2 mass spectra, potassium adducts of the ligand with one (m/z = 508.2, z = 2+), two (m/z = 527.2, z = 2+), three (m/z = 546.2, z = 2+) and four (m/z = 565.1, z = 2+) potassium atoms are observed (Fig. S4†). In the Zn(II)-Ac-LHRFWHLK-NH2 mass spectra, beside the signal from the ligand and zinc complex, a ligand adduct with one (m/z = 608.3, z = 2+) and two potassium atoms (m/z = 627.3, z = 2+) is visible (Fig. S5†). In the case of Zn(II)-Ac-SHQHT-NH2, sodium (m/z = 672.3, z = 1+), potassium (m/z = 688.3, z = 1+) and perchlorate (m/z = 747.2, z = 1+) adducts of the peptide are present (Fig. S6†). In the Zn(II)-Ac-SHCHTHADGEVHC-COOH mass spectra, a signal which is the result of the loss of a water molecule from the peptide during ionisation (m/z = 486.2, z = 3+) and a ligand potassium adduct is visible (m/z = 504.9, z = 3+) (Fig. S7†). In the mass spectra of Zn(II)-Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH, we observe a signal which comes from the loss of a water molecule from the peptide (m/z = 781.1, z = 4+), a peptide sodium adduct (m/z = 791.1, z = 4+), a potassium ligand adduct with one (m/z = 795.1, z = 4+) and two (m/z = 804.8, z = 4+) atoms and a chloride adduct of the zinc complex (m/z = 810.8, z = 4+) (Fig. S8†).
The first complex observed for Ac-AEHARDH-NH2 is ZnH2L with a maximum at pH 4.5. It involves two acidic residues in binding, which are aspartic and glutamic acids. The next two forms, ZnHL and ZnL, with maxima at pH around 5.8 and 7.3, respectively, are both related to the proton loss and coordination of histidine residues. A significant decrease of the pKa values calculated for the complexed and free imidazoles (Table 1) suggests that both histidines are involved in zinc binding. The last two observed forms, ZnH−1L and ZnH−2L, correspond to the deprotonation of two water molecules (Fig. S10†).
Ac-IEHY-NH2 | Ac-AEHARDH-NH2 | Ac-LHRFWHLK-NH2 | Ac-SHQHT-NH2 | Ac-SHCHTHADGEVHC-COOH | Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Species | logβ | pKa | logβ | pKa | logβ | pKa | logβ | pKa | logβ | pKa | logβ | pKa |
HL | 9.93 (3) | 9.93 (Tyr) | 6.97 (5) | 6.97 (His) | 10.29 (1) | 10.29 (Lys) | 6.74 (2) | 6.74 (His) | 9.56 (1) | 9.56 (Cys) | 9.71 (2) | 9.71 (Cys) |
H2L | 16.51 (1) | 6.58 (His) | 13.22 (4 | 6.25 (His) | 16.88 (1) | 6.59 (His) | 12.63 (1) | 5.90 (His) | 17.82 (2) | 8.27 (Cys) | 18.07 (2) | 8.36 (Cys) |
H3L | 20.69 (1) | 4.18 (Glu) | 17.44 (3) | 4.22 (Glu) | 22.71 (2) | 5.83 (His) | 24.95 (2) | 7.13 (His) | 25.35 (4) | 7.28 (His) | ||
H4L | 20.82 (3) | 3.38 (Asp) | 31.65 (2) | 6.71 (His) | 32.55 (4) | 7.20 (His) | ||||||
H5L | 37.83 (2) | 6.18 (His) | 39.31 (4) | 6.75 (His) | ||||||||
H6L | 42.90 (2) | 5.07 (His) | 45.65 (4) | 6.34 (His) | ||||||||
H7L | 46.80 (2) | 3.90 (Glu) | 51.77 (2) | 6.12 (His) | ||||||||
H8L | 49.75 (2) | 2.95 (Asp) | 56.82 (3) | 5.05 (His) | ||||||||
H9L | 60.87 (3) | 4.05 (Glu) | ||||||||||
H10L | 64.63 (3) | 3.75 (Asp) | ||||||||||
H11L | 67.52 (4) | 2.89 (Asp) | ||||||||||
H12L | 70.37 (3) | 2.85 (Asp) | ||||||||||
Zn2+ complexes | ||||||||||||
ZnH6L | 49.72 (5) | |||||||||||
ZnH5L | ||||||||||||
ZnH4L | 39.20 (1) | |||||||||||
ZnH3L | 30.78 (1) | 32.66 (4) | 6.55 (His) | |||||||||
ZnH2L | 19.91 (4) | 17.35 (2) | 21.53 (2) | 24.84 (1) | 5.94 (His) | 26.03(3) | 6.63 (His) | |||||
ZnHL | 13.74 (2) | 6.17 (His) | 12.26 (3) | 5.09 (His) | 16.47 (2) | 5.06 | 15.90 (4) | 8.95 (Cys) | 18.57(3) | 7.46 (Cys) | ||
ZnL | 5.99 (3) | 6.27 (His) | 3.82 (4) | 3.82 (2His) | 5.93 (6) | 9.96 (Cys) | 9.50 (4) | 9.07 (Cys) | ||||
ZnH−1L | −1.99 (2) | −2.19 (2) | 8.18 (H2O) | −0.78 (5) | ||||||||
ZnH−2L | −11.64 (2) | 9.65 | −10.69 (6) | 8.50 (H2O) | −10.74 (6) | 9.96 | −11.96 (3) |
In the case of Ac-LHRFWHLK-NH2, zinc coordination starts at pH 3 and the first form we observe is ZnH2L with a maximum at pH 4.6. It involves one histidine imidazole in binding. The next form which dominates at around physiological pH (max. at 7.3) is ZnHL. The lowered pKa value for the histidine residue in the complexed ligand (5.06) compared to the free one (6.59) implies the participation of this group in binding. The loss of the next two protons leads to ZnH−1L, which indicates the proton loss from two water molecules bound to the central zinc atom. The last observed form, ZnH−2L, which dominates at basic pH, comes from the deprotonation of lysine, which does not participate in coordination (Fig. S11†). Above pH 9, precipitation of the complex starts to occur.
For Ac-SHQHT-NH2, we observe only two complex forms with zinc ions (Fig. S12†). First of them is ZnL with a maximum at pH 7.0. It engages two histidine residues in binding. The remaining two deprotonations are related to the proton loss of water molecules, which are most probably present in the vacant zinc binding sites.
The first complex form of the C-terminal Ac-SHCHTHADGEVHC-COOH that we observe is ZnH3L with a maximum at around pH 5.6 (Fig. S13†). It involves three histidine residues in binding. Above pH 6, the ZnH3L complex deprotonates to ZnH2L with a pKa value of 5.94, which corresponds to the remaining histidine residue, which is also involved in coordination (its pKa in the free ligand = 7.13). The loss of the next two protons corresponds to the deprotonation of two cysteine residues, which do not participate in binding.
The longest examined Pra1 region, Ac-SHQHTDSNPSA-TTDANSHCHTHADGEVHC-COOH, involves the Ac-SHQHT-NH2 and Ac-SHCHTHADGEVHC-COOH fragments. Zinc coordination starts at pH around 4 and the first form, ZnH6L (with a maximum at pH 5.2, Fig. S14†), involves two histidine residues in binding. Around pH 6, the ZnH4L form dominates in solution, with the other two histidine imidazoles being bound to the metal, which by now has a {4N} binding mode. The next two deprotonations with pKa values of 6.55 and 6.63, respectively (corresponding to two deprotonations of unbound histidines), lead to the formation of ZnH3L and ZnH2L forms. The {4N} binding mode does not change in the two last complex forms, ZnHL and ZnL; deprotonations come from the cysteine side chains which, as in the case of the Ac-SHCHTHADGEVHC-COOH fragment, do not take part in coordination.
Establishing which of the six histidines are bound to the metal was not a trivial task, and it seems quite likely that several species with the same stoichiometry but different donor atom sets are present in solution at the same time and at equilibrium with each other.
NMR measurements were performed in order to point out which imidazoles are involved in binding. NOESY and TOCSY experiments were performed at pH 5 and 6 in the presence as well as in the absence of zinc. The histidine Hα–Hβ and aromatic proton correlations are severely overlapped, most likely because the chemical environment around the histidines is very similar (repetitions of ‘SH’ and ‘HT’). Because of this, we were not able to univocally assign signals coming from each histidine. However, the informative part of the spectra was the region of histidine Hβ–Hδ correlations, which broaden to the baseline upon the addition of zinc. Fig. S15† shows that four such correlations are not affected at pH 5 (maximum abundance of ZnH4L species) and two are still visible at pH 6 (maximum of ZnH2L) (Fig. S16†), confirming the information from potentiometric data – two of the six histidines from this fragment bind Zn(II) at pH 5, and four imidazoles are coordinated at pH 6. Again – which ones? There is virtually no difference in the TOCSY spectra of the discussed fragment recorded with and without the presence of zinc, apart from a partial broadening in the region of the overlapped His Hα–Hβ region at around 3.3–4.7 ppm and the broadening of a small resonance at 2.1–4.3 ppm which can be assigned to the Hα–Hβ correlation of Val297, present only before the last His298 in the studied sequence (Fig. S17†). Naturally, this does not imply the engagement of Val297 in coordination, rather the involvement of His298; the resonances of Val297 are affected due to the fact that they sense a different chemical environment after the binding of Zn(II) to His298, which is in close proximity. The hypothesis that the last four C-terminal His residues are involved in the binding is further supported by a competition plot shown in Fig. 3. The plot is based on the calculated constants, showing a hypothetical situation, in which equimolar amounts of Zn(II), Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH, Ac-SHCHTHADGEVHC-COOH and Ac-SHQHT-NH2 are present. The comparison of these stabilities shows that the N-terminal part of Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH-Ac-SHQHT-NH2 is not as effective in binding Zn(II) as the C-terminal Ac-SHCHTHADGEVHC-COOH part. The zinc coordination to Ac-SHCHTHADGEVHC-COOH and Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH is almost identical until pH 6 – at this point, both complexes have a {4N} binding mode. Above pH 6, Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH becomes a more efficient Zn(II) binder. We may hypothesize that this is due either to (i) the formation of polymorphic states (species with the same {4N} binding mode but different His imidazole donors are present in solution at the same time and the metal ion can move along the histidines, as described in ref. 35) or to (ii) the protection of the complex core from hydrolysis by non-binding residues that form a set of hydrogen bonds around the primary coordination sphere (as reported in ref. 36).
Fig. 3 Competition plot between Pra1 fragments: Ac-SHQHT-NH2, Ac-SHCHTHADGEVHC-COOH, Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH and Zn(II), describes complex formation at different pH values in a hypothetical situation in which equimolar amounts of the four reagents are mixed. Calculations are based on binding constants from Table 1. Conditions: 298 K, I = 0.1 M, [Zn(II)] = [Ac-SHCHTHADGEVHC-COOH] = [Ac-SHQHTDSNPSATTDANSHCHTHADGEVHC-COOH] = [Ac-SHQHT-NH2] = 0.001 M. |
Fig. 4 A competition plot between Pra1 fragments: Ac-IEHY-NH2; Ac-AEHARDH-NH2; Ac-SHQHT-NH2; Ac-SHCHTHADGEVHC-COOH and Zn(II), describes complex formation at different pH values in a hypothetical situation in which equimolar amounts of the four reagents are mixed. Calculations are based on binding constants from Table 1. Conditions: 298 K, I = 0.1 M, [Zn(II)] = [Ac-IEHY-NH2] = [Ac-AEHARDH-NH2] = [Ac-SHQHT-NH2] = [Ac-SHCHTHADGEVHC-COOH] = 0.001M. |
At acidic pH, fragments with glutamic and/or aspartic acid residues become the anchoring site for Zn(II). It is not surprising that Ac-AEHARDH-NH2, in which two acidic binding groups participate in binding, is preferred over Ac-IEHY-NH2, with only one acidic binding group.
Interestingly, the Ac-LHRFWHLK-NH2 region, in which two histidine residues are the primary zinc binding sites, starts to coordinate already at pH above 3 (ca. 3% of available zinc is bound to the sequence). The binding was confirmed by NMR (Fig. S18,† an overlay of TOCSY spectra recorded for the fragment in the presence and absence of Zn(II) at pH 3.5 shows selective broadening of aromatic His protons). In fact, Ac-LHRFWHLK-NH2 is the region of choice for Zn(II) at a narrow pH range from 5.4–6.0. This high affinity towards zinc can be explained by the presence of hydrophobic residues (2 × Leu, Phe, Trp) in close proximity to the binding site. Such an enhancement of zinc binding affinity in the presence of hydrophobic residues was previously observed for carbonic anhydrase37 or several transcription factors.38
Above pH 6, the C-terminal, unstructured Pra1 fragment becomes the primary zinc binding site. At around physiological pH (7.4), more than 85% of the metal is coordinated to this sequence, being bound to the imidazole nitrogens of His288, His290, His292 and His298. The proposed coordination mode is depicted in Fig. 5.
Fig. 5 Proposed binding mode for the Zn(II)–Pra1 complex at pH 7.2: (A) view of the whole zincophore; (B) enlarged binding site. Pra1 structure is based on coordinates simulated by Phyre2.28 The figure was generated using PyMOL.39 |
Previous biological studies have shown that Pra1 is a pH-regulated antigen (PRA1 is transcriptionally upregulated at neutral-alkaline pH).23Fig. 4 further confirms the observations made by Citiulo22 and Wilson27 that Pra1's recently described role as a zincophore may also be restricted to the environments of higher pH – Pra1's most efficient zinc binding site coordinates the metal with the highest affinity above pH 7.
Pra1's C-terminal affinity for zinc can also be expressed in terms of affinity constants. Based on the concentrations of the ligand, Zn(II) and the formed complex at pH 7.4 from the simulated speciation in Hyss34 give a 13 nM constant, comparable to that of calprotectin reported by Kehl-Fie.40 We may hypothesise that Pra1 could compete with calprotectin for the binding of Zn(II), making the zincophore-calprotectin struggle for this metal highly interesting in the field of host–pathogen interactions and nutritional immunity.
The fact that Pra1's C-terminal domain is the most probable Zn(II) binding site is also interesting from an evolutionary perspective27 – it seems that this binding site is ancient, present not only in ascomycota, but also in basidiomycetes and chytrids (e.g. the related C-terminal sequence from Ustilago maydis (corn smut fungus, basidiomycete) shares 57.7% identity with the C-terminus studied herein).
The interactions of this biologically crucial region of the Pra1 zincophore, responsible for zinc coordination, with Zrt1, the C. albicans surface zinc transporter, will be the subject of further studies.
From a chemical point of view, the obtained data allow a very precise comparison of complex thermodynamics, but how biologically relevant is it? It is obvious, that the placement of the studied fragments inside a bigger protein, as well as the presence of the protein in the ‘crowded’ environment of the host plasma, has an influence on zinc binding, which might turn out to differ moderately from the one calculated in our in vitro environment of the chosen peptide fragments. However, the careful choice of fragments for this study based on the criteria of (i) being a good zinc binder; (ii) homology with a zincophore from another fungal species (A. fumigatus Aspf2) and (iii) being in an unstructured region of the protein, and therefore appropriate for our peptide-based approach, make this study a biologically relevant one.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c7dt01675a |
This journal is © The Royal Society of Chemistry 2017 |