Issue 22, 2017

A simple and efficient fluorescent probe for the rapid detection of H2S in living cells and on agar gels

Abstract

Herein, a simple fluorescent probe, (E)-1,3,3-trimethyl-2-(4-morpholinostyryl)-3H-indol-1-ium iodide (Mi), based on intramolecular charge transfer (ICT) was synthesized. The probe can rapidly and sensitively sense hydrogen sulphide (H2S) with low detection limit (15 nM). The reaction mechanism was investigated via fluorescence and mass spectra. Moreover, the Mi probe was successfully used for the fluorescent imaging of H2S in living cells, demonstrating its potential applications in biological science. Finally, agar gels based on the Mi probe were fabricated, which could act as a solid optical sensor to conveniently and efficiently detect H2S.

Graphical abstract: A simple and efficient fluorescent probe for the rapid detection of H2S in living cells and on agar gels

Supplementary files

Article information

Article type
Paper
Submitted
24 Feb 2017
Accepted
27 Apr 2017
First published
04 May 2017

Anal. Methods, 2017,9, 3290-3295

A simple and efficient fluorescent probe for the rapid detection of H2S in living cells and on agar gels

Y. Li, B. Gu, W. Su, X. Duan, H. Xu, Z. Huang, H. Li and S. Yao, Anal. Methods, 2017, 9, 3290 DOI: 10.1039/C7AY00499K

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