Issue 27, 2017

Gold nanoparticles stabilized by cationic carbosilane dendrons: synthesis and biological properties

Abstract

Gold nanoparticles (AuNPs) and polycationic macromolecules are used as gene carriers. Their behaviour is dependent on several factors, such as the size and type of the framework, charge, etc. We have combined both types of systems and prepared AuNPs covered with cationic carbosilane dendrons with the aim to evaluate their biocompatibility. Water soluble dendronized cationic AuNPs were prepared following a straightforward procedure from dendrons, a gold precursor and a reducing agent in water and were characterized by 1H NMR, transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), ultraviolet spectroscopy (UV), and zeta potential (ZP). The biological properties of dendrons and AuNPs were determined by hemolysis, platelet aggregation and lymphocyte proliferation. These assays reflect modification of dendron properties when covering nanoparticles. For dendrons, hemolysis and platelet aggregation are generation dependent whilst, for AuNPs these properties are related to the bigger size of NPs. On the other hand, none of the systems induced lymphocyte proliferation. Selected cationic dendrons and AuNPs were chosen for gene delivery experiments employing a small interference RNA (siRNA Nef) against human immunodeficiency virus (HIV).

Graphical abstract: Gold nanoparticles stabilized by cationic carbosilane dendrons: synthesis and biological properties

Supplementary files

Article information

Article type
Paper
Submitted
29 Sep 2016
Accepted
15 Dec 2016
First published
16 Dec 2016

Dalton Trans., 2017,46, 8736-8745

Gold nanoparticles stabilized by cationic carbosilane dendrons: synthesis and biological properties

C. E. Peña-González, E. Pedziwiatr-Werbicka, D. Shcharbin, C. Guerrero-Beltrán, V. Abashkin, S. Loznikova, J. L. Jiménez, M. Á. Muñoz-Fernández, M. Bryszewska, R. Gómez, J. Sánchez-Nieves and F. J. de la Mata, Dalton Trans., 2017, 46, 8736 DOI: 10.1039/C6DT03791G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements