Quantum dots-based fluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples

Abstract

Quantum dots (QDs), luminescent semiconductor nanocrystals, have the potential to simplify the performance of multi-analyte analysis. In this work, a novel indirect competitive fluorescence-linked immunosorbent assay (FLISA) based on QDs for the detection of clothianidin and thiacloprid simultaneously in environmental and agricultural samples were performed in a single well of the microtiter plate. The QDs-labeled antibody conjugates, which consists of CdSe/ZnS core–shell QDs and polyclonal antibodies, were prepared through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling method. Under optimal conditions, the half-maximal inhibition concentration (IC₅₀) and the limit of detection (LOD, IC₁₀) of the FLISA were 12.5 and 0.3 ng mL⁻¹ for clothianidin, and 9.27 and 0.4 ng mL⁻¹ for thiacloprid, respectively. The cross-reactivities of the FLISA with the analogues of clothianidin and thiacloprid were negligible except for dinotefuran (10.4% for clothianidin). The spiked recoveries were 73.3% to 113.1% with relative standard deviations (RSDs) of 2.7 to 13.3% for the detection of clothianidin and thiacloprid in river water, soil, cabbage, rice and tomato. Furthermore, the results of FLISA for the authentic samples correlated well with those obtained by HPLC. The proposed FLISA is a satisfactory tool for rapid, sensitive, and quantitative detection of two pesticides simultaneously in agricultural and environmental samples. This study provides a strategy for the development of QDs-based fluoroimmunoassay for the quantitative analysis of two pesticides.

---

1. Introduction

In the comprehensive management of biological disasters, pesticides are still the most convenient, effective and inexpensive means for protection of crops.¹ Due to the irrational use of multiple pesticides, the pesticide multi-residues in the environment and food have been noticed. To enhance detecting speed and reduce testing costs, multi-residue analysis has gradually become a research hotspot in residue analysis. The development of simple, rapid, and economical methods to detect two or more pesticides simultaneously would be more useful and significant.²

Currently analytical methods for the determination of pesticide are mainly high performance liquid chromatography (HPLC) and gas chromatography (GC), which are characterized by high precision and sensitivity, but time-consuming, expensive and unsuitable for screening purposes. Immunoassays are increasingly considered as alternative or complementary methods for pesticide residue analysis with significant advantages, such as high specificity, rapidity, simplicity, cost-effective, and high throughput. Immunoassays are often developed to detect a single analyte³ and can also be applied to detect various compounds in a single test.⁴ Up to now, many efforts have been concentrated on developing multi-residue enzyme-linked immunosorbent assays (ELISA), which based on more than one antibody,⁵ general-structure hapten antibody,⁶ multi-hapten antigen antibody⁷ or bispecific monoclonal antibody.⁸ However, vast majority of reported studies were difficult to achieve simultaneous detection of multi-analytes. This fact becomes the greatest question for producing the advantage of multi-analyte analysis.

The fluorescence-linked immunosorbent assay (FLISA), which is based on the use of novel QDs as the fluorescent markers, is one of the most promising multi-analyte immunoassays.⁹ The unique properties of QDs make them an ideal label for multiplex labeling scenarios. The QDs provide the benefit of a good quantum yield, broad excitation spectra, narrow emission peaks, high photostability, and excellent biological compatibility.¹⁰ Moreover, the QDs are robust inorganic fluorophores that exhibit size-dependent that the fluorescence emission wavelength could be tunable by changing the nanocrystal size; hence, simultaneous multi-analyte analysis can be achieved easily using various sizes of QDs in the single test. The QDs-based fluoroimmunoassay have been introduced into detecting of multi-analyte analysis simultaneously in recent years. Goldman et al. performed a FLISA for the quantitation of four toxins.¹¹ Hu et al. developed a QDs-based sandwich fluoroimmunoassay for detecting multi-cancer biomarkers.¹²

Clothianidin and thiacloprid, two promising neonicotinoid insecticides, have been broadly used for the long-term control of a wide variety of pests with excellent efficacies.^13,14 The development of an immunoassay for simultaneous detection of them would be made that the screening of these two pesticides become simple, efficient and labour-saving. There was no FLISA for the separate or simultaneous detection of them.

The present work aims to use QDs-conjugated antibodies to demonstrate that multi-residue fluoroimmunoassay for two pesticides present in environmental and agricultural samples (Fig. 1). For this purpose, anti-clothianidin and anti-thiacloprid antibodies were coupled to CdSe/ZnS core–shell QDs with maximum emission wavelengths at 602 and 510 nm. Moreover, the FLISA has been applied to authentic samples and validated by HPLC.

Fig. 1 Schematic illustration of the FLISA for the simultaneous detection of clothianidin and thiacloprid.

2. Materials and methods

2.1. Reagents and equipments

Clothianidin (97.6% purity), thiacloprid (98.0% purity) and the pesticide standards used for cross-reactivity studies were supplied by Jiangsu Pesticide Research Institute (Jiangsu, China). Bovine serum albumin (BSA), ovalbumin (OVA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and other chemical reagents were purchased from Aladdin (Shanghai, China). The carboxylic groups-modified CdSe/ZnS core–shell QDs (having maximum emission wavelengths at 602 and 510 nm) were purchased from Wuhan Jiayuan Quantum Dots Co., Ltd. (Wuhan, China). Carbonate-buffered saline buffer (CBS, 0.05 mol L⁻¹, pH 9.6), sodium borate buffer (BB, 0.05 mol L⁻¹, pH 9.0), and BB containing 0.05% Tween-20 (BBT) were used. The haptens (Fig. 2), coating antigens and polyclonal antibodies were stored in the laboratory.^15,16

Fig. 2 Structures of clothianidin, thiacloprid and their haptens.

Black microplates (96-well) were obtained from Corning Costar Corporation (New York, USA). Washing steps were carried out using a Wellwash Plus (Thermo, USA). The fluorescence was detected using a SpectraMax M5 (Molecular Devices, Sunnyvale, USA). The centrifugation was performed on an Allegra TM 64R Centrifuge (Beckman, USA). The results of FLISA were validated with Aglient 1260 HPLC equipped with ultraviolet detector (Aglient, Wilmington, USA).

2.2. Preparation of the QDs-labeled antibodies

The conjugates of QDs-labeled antibodies were prepared by using the EDC coupling method.¹⁷ Briefly, after being dialyzed against BB, the concentrations of C-PcAb and T-PcAb were adjusted to 5 mg mL⁻¹ using the BB. A volume of 270 μL of C-PcAb was mixed with 30 μL of QDs602 in a small amber bottle. Then 15 μL of freshly prepared 10 mg mL⁻¹ EDC solution was quickly added to the mixture. Thereafter, the mixture was reacted at room temperature for 2 h under continuous stirring in the dark. The conjugate of QDs602/C-PcAb was purified by centrifugation for 30 min at 18 000 rpm at 4 °C. The centrifugal speed and time should be optimized to ensure that the conjugate of QDs602/C-PcAb was separated from the mixture and the free QDs602 and C-PcAb remained in the liquid. The supernatants were carefully removed, and the loose sediments were resuspended in 300 μL of BB. The centrifugation was repeated twice to completely remove the unconjugated QDs602 and C-PcAb. Finally, the sediment of QDs602/C-PcAb were resuspended in 500 μL of BB (containing 1% BSA and 0.05% sodiumazide) and stored at 4 °C. The conjugate of QDs510/T-PcAb was prepared the same as the above-mentioned.

2.3. Development of FLISA

Procedures of FLISA. The schematic diagram of the QDs-based FLISA procedure for the simultaneous detection of clothianidin and thiacloprid was showed in Fig. 1. Ninety-six-well black microplates were coated with the mixture of the two coating antigens (100 μL per well, in CBS) overnight at 4 °C. The plates were washed five times with BBT and blocked by incubating with 1% OVA in BB (200 μL per well) for 0.5 h at 37 °C. After another washing step, either the samples or mixtures of standard serial concentrations of clothianidin and thiacloprid in BB containing methanol (50 μL per well) were added followed by addition of the optimal mixtures of QDs602/C-PcAb and QDs510/T-PcAb dilution (50 μL per well, in BB) together for 1 h at 37 °C. Then the plates were washed again and padded dry. The fluorescence intensity (F) was measured with a SpectraMax M5 (excitation wavelength at 260 nm; emission wavelength at 606 nm for clothianidin and 516 nm for thiacloprid). Determinations were carried out in triplicate, and the mean values of F/F₀ (F: the fluorescence signal with analytes; F₀: the fluorescence signal absence of analytes) were plotted against the concentration of analytes to obtain the standard curves using Origin Pro 7.0 software.

Optimization of experimental parameters. The experimental parameters (concentrations of the coating antigen and antibody, organic solvent, ionic strength, and pH) were studied to improve the sensitivity of the FLISA. The solutions with series concentrations of analytes and varied contents of methanol, ionic strength or pH were tested. The F₀/IC₅₀ ratio and the IC₅₀ were used as primary criterions to evaluate FLISA performances, the highest ratio of F₀/IC₅₀ and the lowest of IC₅₀ were the most desirable.

Cross-reactivities. The specificity of the FLISA was evaluated by testing the cross-reactivity (CR) of antibodies with the analogues of clothianidin and thiacloprid under optimized conditions. The CR values were calculated as follows:

CR% = (IC₅₀ of analyte/IC₅₀ of analogue) × 100

2.4. Analysis of spiked samples by FLISA

The river water, soil, cabbage, rice and tomato samples that had been certified as free of clothianidin and thiacloprid were used for the matrix effect and recovery studies. The filtered water samples (10 mL) were spiked with clothianidin and thiacloprid at 10, 50, 500 ng mL⁻¹ and stored overnight, which were directly analyzed by FLISA. The homogenized samples of soil, cabbage, rice and tomato were finely chopped. These samples (10 g) were added clothianidin and thiacloprid at concentrations of 10, 50, 500 ng mL⁻¹ and stored overnight. The samples were mixed with 10 mL of BB containing 20% methanol, submitted to ultrasonic extraction for 10 min, and then centrifuged for 10 min at 4000 rpm. The supernatants were diluted and adjusted to the optimized working solution condition, and then the concentrations of clothianidin and thiacloprid were simultaneously analyzed using the optimum FLISA. Each analysis was performed in triplicate. The accuracy and precision of FLISA were evaluated by the recoveries of the clothianidin and thiacloprid and relative standard deviations (RSDs).

2.5. Evaluation of authentic samples by FLISA and HPLC

The mixture of 40% clothianidin wettable powder and 48% thiacloprid suspension was sprayed onto paddy fields. Paddy water, soil and rice samples were then picked randomly and analyzed with FLISA and HPLC. For HPLC, the paddy water samples were extracted twice with ethyl acetate, and the soil and rice samples were extracted by vigorously shaking for 1 h with acetonitrile. After the organic phase was dehydrated and concentrated, the samples were dissolved with 2 mL of methanol and further confirmed by HPLC¹⁸ with an Eclipse XDB2-C18 column (250 mm × 4.6 mm × 5 μm) using a mixture of acetonitrile and water (30 : 70, v/v) as the mobile phase at a flow rate of 1.0 mL min⁻¹ at 30 °C. The detection wavelength was 265 nm for clothianidin and 245 nm for thiacloprid, and the injection volume was 20 μL.

3. Results and discussion

3.1. Fluorescent features of the QDs-labeled antibodies

The CdSe/ZnS core–shell QDs conjugated with antibodies were prepared and characterized. It showed that the emission spectra of QDs-labeled antibodies appeared shifted towards the longer wavelength sides (Fig. S1†). Besides, the following FLISA indicated that the QDs-labeled antibodies were coupled successfully. The narrow and symmetric emission spectral bandwidths were shown in Fig. S2.† These optical qualities can minimize channel overlap, improve color discrimination, and enable multi-analyte analysis specifically. Thus, the QDs-labeled antibodies might meet the requirement for developing multi-analyte FLISA. It would be nearly impossible to do a similar experiment for detecting two pesticides simultaneously in a single well using ELISA due to the spectral characteristics of enzyme (same singles of the enzyme tracers). It can only determine the gross of analytes using the ELISA in a single well.

During 2 months of storage, the sensitivity and stability of the QDs-labeled antibodies did not show significant difference. This indicated that the QDs-labeled antibodies (in BB containing 1% BSA and 0.05% sodiumazide) could be stored at least 2 months at 4 °C.

3.2. Optimization of FLISA conditions

The concentration of coating antigens, and QDs-labeled antibodies were optimized base on the higher F₀/IC₅₀ ratio and lower IC₅₀. The optimal concentrations of coating antigens were 4000-fold dilution for clothianidin and 2000-fold dilution for thiacloprid. And the optimal concentrations of QDs602/C-PcAb and QDs510/T-PcAb were 400-fold dilution.

Organic solvent, ionic strength and pH were investigated to optimize the FLISAs (Fig. S3†). The methanol was selected to improve solubility of analytes and evaluate its effect on the FLISA. The values of F₀/IC₅₀ tended to decrease with the increase of methanol, while the IC₅₀ values showed drastic increase above 10% methanol for clothianidin and above 5% methanol for thiacloprid. For detecting clothianidin and thiacloprid simultaneously, the methanol concentrations being 5% was selected as the optimum condition. The change of Na⁺ concentration from 0.1 to 0.6 mol L⁻¹ also influenced the FLISA dramatically. The sensitivity of the FLISA was improving with the increasing of Na⁺. The highest F₀/IC₅₀ and lowest IC₅₀ were acquired at 0.4 mol L⁻¹ Na⁺ for these analytes. In addition, the pH did not have a notable effect on the sensitivity of the FLISA. On the basis of these results, 5% methanol, 0.4 mol L⁻¹ Na⁺ and pH 7.4 were chosen as the optimal conditions for the FLISA.

3.3. Sensitivities

The standard curves of clothianidin and thiacloprid were constructed under the optimum conditions (Fig. 3). The FLISA for clothianidin was shown to have an IC₅₀ of 12.5 ng mL⁻¹, a LOD of 0.3 ng mL⁻¹, and a linear range (IC₁₀–IC₉₀) of 0.3–521 ng mL⁻¹. For thiacloprid, the IC₅₀ value, LOD value and linear range were 9.27, 0.4, and 0.4–192 ng mL⁻¹, respectively.

Fig. 3 FPIA calibration curves for clothianidin and thiacloprid.

The maximum residue limits (MRLs) of clothianidin were set at 10 ng g⁻¹ for sorghum, and 50 ng g⁻¹ for potato in the USA.¹⁸ The MRLs of thiacloprid were set at 20 ng g⁻¹ for rice and onion in the UK.¹⁹ Compared to the MRLs of clothianidin and thiacloprid, the sensitivity of the FLISA could meet the requirements for detecting clothianidin and thiacloprid residues. Using the same antibody, the IC₅₀ and LOD of ELISA were 46 and 2.8 ng mL⁻¹ for clothianidin,¹⁵ and 10 and 0.47 ng mL⁻¹ for thiacloprid.¹⁶ Comparison of the conventional ELISAs, the sensitivity of the developed FLISA has a significantly improvement for clothianidin and slightly increased for thiacloprid. In addition, the LOD of GC and HPLC were 10 and 4 ng mL⁻¹ for clothianidin,^20,21 and the LOD of HPLC were 8 ng mL⁻¹ for thiacloprid.²² To sum up, the proposed FLISA showed higher sensitivity than the reported ELISAs and instrument-based detection methods.

3.4. Specificity

The FLISA showed negligible CRs with analogues (Fig. 4) except dinotefuran, one of N-methyl-N′-nitroguanidine analogues (exhibited higher CR for clothianidin detection, 10.4%). These results were consistent with the CR results obtained using the ELISA format previously,^15,16 which verified the highly specific of the FLISA for clothianidin and thiacloprid.

Fig. 4 Cross-reactivity of clothianidin and thiacloprid toward their analogues in FLISA.

3.5. Accuracy and precision

The matrix effects are one of the most common challenges in performing immunoassays on complex samples. In this study, the matrix effects have been minimized by dilution methods, and the schemes of dilution were showed in Table 1. The matrix interference was negligible in the water samples, which could be analyzed directly without dilution. Moreover, the matrix interference could be negligible when the matrixes were five-fold dilution for the soil samples, and ten-fold dilution for the cabbage, rice and tomato samples. The schemes of dilution were applied for subsequent experiments.

Table 1 Accuracy and precision of clothianidin and thiacloprid by FLISA

Sample	Spiked concentration (ng g^−1)	Dilution times	Clothianidin		Thiacloprid
			Mean recovery (%, n = 3)	RSD (%)	Mean recovery (%, n = 3)	RSD (%)
Water	10	0	79.2	6.5	88.1	5.7
	50		85.4	3.7	98.4	7.3
	500		86.7	3.9	95.2	4.6
Soil	10	5	113.1	7.2	86.1	3.3
	50		101.7	3.2	84.3	8.3
	500		95.2	5.4	83.7	4.1
Cabbage	10	10	73.3	11.2	85.6	5.5
	50		87.0	8.3	91.4	8.3
	500		94.5	3.5	90.3	5.6
Rice	10	10	97.3	4.8	107.4	7.6
	50		106.4	3.3	106.7	13.3
	500		94.6	6.0	94.6	6.3
Tomato	10	10	90.2	3.1	83.2	3.7
	50		92.9	5.5	84.0	2.7
	500		89.4	3.1	93.1	4.2

---

As illustrated in Table 1, the recoveries of clothianidin for FLISA ranged from 73.3 to 113.1% with the RSDs between 3.1 to 11.2%, and the recoveries of thiacloprid were from 83.2 to 107.4% with the RSDs between 2.7 to 13.3%. These results indicated that the accuracy and precision of the developed FLISA was satisfactory for the qualitative and quantitative determination of clothianidin and thiacloprid simultaneously in agricultural and environmental samples.

3.6. Correlation of FLISA and HPLC

The FLISA showed that the authentic samples of paddy water, soil and rice were tested as positive (Table 2), which ranged from 13.1 to 601.6 ng g⁻¹ for clothianidin and 9.6 to 574.3 ng g⁻¹ for thiacloprid. The subsequent HPLC gave largely consistent results as with FLISA, where the positive results ranged from 15.2 to 581.4 ng g⁻¹ for clothianidin and 11.4 to 598.6 ng g⁻¹ for thiacloprid. As shown in Fig. 5, good correlations between the FLISA (y) and HPLC (x) for the authentic samples was obtained (for clothianidin: y = 0.9812x − 0.6461, R² = 0.9969; for thiacloprid: y = 1.016x − 3.3232, R² = 0.9962), which further demonstrated the results of FLISA detection were reliability.

Table 2 Detection of clothianidin and thiacloprid residues in the authentic samples by FLISA and HPLC

Sample		Clothianidin		Thiacloprid
		Mean ± SD (ng g^−1)		Mean ± SD (ng g^−1)
		FLISA	HPLC	FLISA	HPLC
1	Paddy water	511.6 ± 18.0	527.1 ± 19.7	492.7 ± 22.1	472.3 ± 15.6
2		211.5 ± 16.5	194.8 ± 13.3	183.2 ± 9.6	165.6 ± 10.3
3		47.2 ± 6.1	54.4 ± 7.5	72.6 ± 8.7	68.5 ± 5.2
4	Soil	601.6 ± 19.2	581.4 ± 22.6	574.3 ± 22.5	598.6 ± 17.7
5		378.4 ± 20.1	355.5 ± 12.8	412.4 ± 12.3	425.1 ± 9.4
6		94.3 ± 6.9	87.5 ± 7.4	114.2 ± 10.7	122.6 ± 12.3
7		27.7 ± 3.6	31.2 ± 5.3	54.2 ± 2.8	48.4 ± 4.5
8	Rice	32.7 ± 2.5	29.2 ± 1.5	21.6 ± 2.4	23.3 ± 3.1
9		13.1 ± 1.5	15.2 ± 0.8	9.6 ± 3.9	11.4 ± 2.2

---

Fig. 5 Correlation between the FLISA and HPLC for detecting clothianidin (A) and thiacloprid (B) in authentic samples.

4. Conclusions

In summary, a novel indirect competitive FLISA for simultaneous detection of clothianidin and thiacloprid was successfully developed by using QDs as tracers. The sensitivities of the FLISA were improved appreciably. Moreover, the developed FLISA shortens the overall analytical procedure, testing time and workload compared to the ELISAs and instrument-based detection methods. The accuracy and precision of the FLISA met the requirements of residue analysis. The authentic samples analysis demonstrated the FLISA was an advantageous analytical method in clothianidin and thiacloprid monitoring. With respect to the developed FLISA's rapidity, sensitivity, lower expenses and high-throughput, the clothianidin and thiacloprid residue in agricultural and environmental samples might be detected simultaneously in a single well. This study provides strategy for the development of QDs-based fluoroimmunoassay for the simultaneous analysis of two pesticides in different samples. In the future studies, QDs-based fluoroimmunoassay should be expandable to assay more pesticides simultaneously, thus the determination of pesticide multi-residue will be more rapid, simple, convenient and efficient.

Acknowledgements

This work was supported by the National “863” High-Tech Research Program of China (2011AA100806) and the Doctoral Program of Higher Education Research Fund (20130097120006).

References

1. Y. H. Wang, Y. W. Jiang and H. Cheng, Mod. Sci. Instrum., 2003, 1, 8–12.
2. H. Y. Zhang, S. Wang and G. Z. Fang, J. Immunol. Methods, 2011, 368, 1–23.
3. Y. L. Yuan, X. D. Hua, M. Li, W. Yin, H. Y. Shi and M. H. Wang, RSC Adv., 2014, 4, 24406–24411.
4. G. F. Lin, H. Zhao, T. C. Liu, J. J. Hou, Z. Q. Ren, Y. S. Wu, W. Q. Dong and W. H. Huang, RSC Adv., 2014, 4, 55229–55236.
5. J. W. Sun, T. T. Dong, Y. Zhang and S. Wang, Anal. Chim. Acta, 2010, 666, 76–82.
6. X. D. Hua, J. F. Yang, L. M. Wang, Q. K. Fang, G. P. Zhang and F. Q. Liu, PLoS One, 2012, 7, e53099.
7. X. Yan, H. Y. Shi and M. H. Wang, Anal. Methods, 2012, 4, 4053–4057.
8. X. D. Hua, L. M. Wang, G. Li, Q. K. Fang, M. H. Wang and F. Q. Liu, Anal. Methods, 2013, 5, 1556–1563.
9. B. M. Lingerfelt, H. Mattoussi, E. R. Goldman, J. M. Mauro and G. P. Anderson, Anal. Chem., 2003, 75, 4043–4049.
10. J. X. Chen, F. Xu, H. Y. Jiang, Y. L. Hou, Q. X. Rao, P. J. Guo and S. Y. Ding, Food Chem., 2009, 113, 1197–1201.
11. E. R. Goldman, A. R. Clapp, G. P. Anderson, H. T. Uyeda, J. M. Mauro, I. L. Medintz and H. Mattoussi, Anal. Chem., 2004, 76, 684–688.
12. M. Hu, J. Yan, Y. He, H. T. Lu, L. X. Weng, S. P. Song, C. H. Fan and L. H. Wang, ACS Nano, 2010, 4, 488–494.
13. T. Motohiro and Y. C. John, Annu. Rev. Pharmacol. Toxicol., 2005, 45, 247–268.
14. H. Uneme, J. Agric. Food Chem., 2011, 59, 2932–2937.
15. M. Li, E. Z. Sheng, L. J. Cong and M. H. Wang, J. Agric. Food Chem., 2013, 61, 3619–3623.
16. Z. J. Liu, M. Li, H. Y. Shi and M. H. Wang, Food Anal. Method., 2013, 6, 691–697.
17. Y. P. Chen, H. L. Ren, N. Liu, N. Sai, X. Y. Liu, Z. Liu, Z. X. Gao and B. A. Ning, J. Agric. Food Chem., 2010, 58, 8895–8903.
18. M. F. Chen, J. W. Huang, S. S. Wong and G. C. Li, J. Food Drug Anal., 2005, 13, 279–283.
19. J. Saimandir and M. Gopal, Am. J. Plant Sci., 2012, 3, 214–227.
20. L. Li, G. G. Jiang, C. Y. Liu, H. W. Liang, D. L. Sun and W. Li, Food Control, 2012, 25, 265–269.
21. B. M. Kim, J. S. Park, J. H. Choi, A. M. El-Aty, T. W. Na and J. H. Shim, Food Chem., 2012, 131, 1546–1551.
22. L. D. Liu, Z. G. Hou, L. D. Yin, C. Chen, Z. B. Lu and W. M. Xie, J. Anhui Agric. Sci., 2010, 38, 12556–12558.

---

Footnote

† Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra13305f

---