Issue 52, 2015
From the journal:

Chemical Communications

Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification†

Bao-Zhu Chi,a   Ru-Ping Liang,a   Li Zhanga  and  Jian-Ding Qiu*a  

* Corresponding authors

a Department of Chemistry, Nanchang University, Nanchang 330031, People's Republic of China
E-mail: jdqiu@ncu.edu.cn
Fax: +86 791 83969518
Tel: +86 791 83969518

Abstract

This assay, termed branched cascade enzymatic amplification (BCEA), can be a novel and straightforward method for sensitive and specific microRNA detection in crude cellular extracts of cancer cells at physiological temperature, by coupling two ordinary polymerases, Klenow fragment exo− and terminal deoxynucleotidyl transferase.

Graphical abstract: Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC02864G
Article type
Communication
Submitted
07 Apr 2015
Accepted
18 May 2015
First published
18 May 2015

Chem. Commun., 2015,51, 10543-10546

Permissions

Request permissions

Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification

B. Chi, R. Liang, L. Zhang and J. Qiu, Chem. Commun., 2015, 51, 10543 DOI: 10.1039/C5CC02864G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements