A fluorescent exciplex of thiazole orange (TO) is formed in a single-dye conjugated DNA i-motif. The exciplex fluorescence exhibits a large Stokes shift, high quantum yield, robust response to pH oscillation and little structural disturbance to the DNA quadruplex, which can be used to monitor the folding of high-order DNA structures.
B. Xu, X. Wu, E. K. L. Yeow and F. Shao, Chem. Commun., 2014, 50, 6402 DOI: 10.1039/C4CC01147C
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