Issue 10, 2013
From the journal:

Nanoscale

Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores†

Luca Guerrini,‡*a   Lee Barrett,a   Jennifer A. Dougan,§a   Karen Fauldsa  and  Duncan Graham*a  

* Corresponding authors

a Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1YL, UK
E-mail: luca.guerrini@ctqc.org, duncan.graham@strath.ac.uk

Abstract

A novel fluorescent-based method for characterisation of oligonucleotide–nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.

Graphical abstract: Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores

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Article information

DOI
https://doi.org/10.1039/C3NR01197F
Article type
Communication
Submitted
08 Mar 2013
Accepted
04 Apr 2013
First published
10 Apr 2013

Nanoscale, 2013,5, 4166-4170

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Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores

L. Guerrini, L. Barrett, J. A. Dougan, K. Faulds and D. Graham, Nanoscale, 2013, 5, 4166 DOI: 10.1039/C3NR01197F

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