Piotr M. Korczyk*ab,
Ladislav Derzsic,
Sławomir Jakiełac and
Piotr Garstecki*c
aInstitute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5B, 02-106 Warsaw, Poland. E-mail: piotr.korczyk@ippt.gov.pl
bIan Wark Research Institute, University of South Australia, Mawson Lakes Campus, South Australia, 5095, Australia
cInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. E-mail: garst@ichf.edu.pl
First published on 31st July 2013
We present microfluidic modules (traps) that allow us to lock, shift, dose and merge micro-aliquots of liquid precisely. The precision is hard-wired into the geometry of the device: small values of the capillary number guarantee reproducibility of operation over a range of rates of flow that need not be controlled precisely. The modules can be integrated into systems that perform complicated protocols on micro-droplets while not requiring precision in forcing the flow.
The requirement for precise control of flow is inconvenient in multiple applications, especially point-of-care or simple laboratory testing. As well as in many other spheres of life and technology, diagnostics in emergency-, screening- and in-field-testing often call for protocols to be flexible for the sake of simplicity and robustness of use. A prime example is the well-known strip test that performs only a single prescribed function (assay). This clear functional limitation is rewarded with robustness—the system is very simple to operate and it is very difficult to accidentally cause its malfunction. The development of systems that would be similarly simple to operate yet could allow a wider range of liquid handling protocols to be incorporated into the chips has been one of the significant motivations for the development of microfluidics. Many successful approaches exist, e.g. paper based microfluidics12,13 that expand on strip test technology, slip chips14 which allow one or two sets of operations to be performed in parallel on small portions of liquids, or geometric modules for select operations on microdroplets.15–28 However the challenge of building a platform that would allow all elementary operations to be performed on micro aliquots of liquids and for integration of the modules that perform these operations into functional, hard-wired networks is still to be explored.
From an engineering perspective, it is possible to construct devices that are insensitive to a set of parameters by designing processes in which the parameters to be disregarded are dominated by forces which enforce a well defined state (either static or dynamic). In the strip test this goal is achieved by a gradation of forces: the capillary forces that are stronger than the viscous dissipation guaranteeing filling of the strip up to its volumetric capacity, while viscous resistance to flow dominates inertia and gravity to protect the liquid from random displacement on the strip. In droplet microfluidics such an opportunity is given by the typically small values of the capillary number (Ca) that represents the large mismatch between the large Laplace pressure and small shear, and by the strong dependence of pressure gradients on the cross-section of the microfluidic channels. As both of these effects are strongly dependent on the geometry of the channels and because the geometry of the devices can be very well controlled at the stage of fabrication, the dominating effects of interfacial tension can be used to yield reproducibly area-minimizing shapes of the interface, largely irrespective of the (comparitively small) magnitude of shear stresses. Here we show that it is possible to construct devices operating at low values of Ca for precise operation on droplets, largely irrespective of the parameters that typically need to be precisely controlled, such as: i) the rate of flow, ii) time of switching the flow, or iii) synchronization of inputs.
We use droplets suspended in a continuous liquid that preferentially wets the walls of the microchannels. The channels confine the droplets: drops larger than the largest sphere inscribed into the lumen of the channel are squeezed; the tightness of the channel sets the curvature of the interface and the pressure jump across the interface. Proper design of the geometry of the channel can be thus used to determine the pressure drop along the droplet needed to drive it through a constriction. Second, droplets flowing in channels experience a non-trivial flow of the continuous liquid around them.29 In rectangular channels, small drops (of length comparable to the width of the channel) flow faster than the mean speed of the carrier liquid.30–32 Larger droplets confine the flow of continuous liquid to thin films on the faces of the walls and to gutters in the corners of the lumen. As a result, these longer drops may flow at speeds different than the mean speed of the continuous liquid which may pass by the droplet. As we show below, appropriate design of ‘gutters’ (or ‘bypasses’) allow the effect to be enhanced and to deliberately lock a desired droplet in place while allowing the continuous liquid to pass by.
Below we first introduce the design and operation of the traps and then we show how these traps can be integrated into systems of increasing complexity. As a model application we use the challenge of generating a geometric series of concentrations of a sample in well defined micro-aliquots of liquid without the need to exercise precise control over the flow on the chip.
Fig. 1 Architecture of the metering trap (a) and micrographs illustrating its operation. b) When a droplet of length larger than the length of the trap passes through, the metering trap locks a well defined volume (length) while releasing the excess volume of the droplet downstream. c) When a droplet flows through a filled trap the two droplets merge, exchange masses and the trap releases the excess volume leaving again just a volume precisely defined by the geometry of the trap. d) Micrographs illustrating the mass exchange between two dyed droplets. After the excess volume is released the trapped droplet is mixed via circulation induced by the flow of the continuous phase. Note, that since the trap has a barrier only on one end, by reversing the flow the droplet can be released for further processing. The width of the straight segments of the channel is 400 μm. |
The metering trap can also be used to generate a sequence of serial dilutions in a procedure very similar to the one presented first by Niu et al.15 Once a droplet with the sample is locked, passing a drop of solvent will i) merge it with the sample, and ii) push out an equal volume of the sample out (Fig. 1d). The flow of the continuous liquid through the bypasses stirs the content of the trapped droplet and speeds up mixing of its content. Passing a sequence of droplets of buffer through the metering trap initially containing a droplet of the sample, generates a sequence of dilutions. If the time between two consecutive titrations is longer than the time required for homogenizing the solution in the trap, the sequence presents an exponential (serial dilution) sequence of concentrations (see Fig. 2).
Fig. 2 Generation of a serial dilution sequence from a metering trap by passing through a series of droplets from a DOD module.11 Data shown on the graph were obtained by analysing images from four independent measurements. Red line indicates best power fit (f(x) = 1.5 × 10−0.39x, where x is the droplet number, and the inset shows a sequence of snapshots of the consecutive dilutions. The width of the straight segments of the channel is 400 μm. |
Fig. 3 Merging trap. a) Scheme of the trap. b) Droplets longer than length of the trap passes through it without stopping or slowing down. c) Droplets shorter than the length of the trap lock in, while the carrier liquid flows around it via the bypasses. d) When a droplet flows into a filled trap the two droplets merge. If the residual droplet is longer than the trap it completely releases the trap, while the content of the two initial droplets mixes effectively even in straight channels. The width of the straight segments of the channel is 400 μm. |
The operation of the merging trap can be described as follows. A droplet longer than the trap (Fig. 3b) passes through it because it continues to block the inlets to the bypasses when its front meets the intrusions at the outlet of the trap. A droplet shorter than the trap (Fig. 3c) is locked once its front approaches the side intrusions at the outlet of the trap because the continuous liquid flows by via the bypasses. When a second droplet flows into the trap, it flows all the way down to the first drop because the continuous liquid can be expelled from in-between the two drops via the bypasses. This allows the drops to merge. If the merged droplet is shorter than the trap it will remain trapped. If it is longer than the trap its rear blocks the entrance to the bypasses which leads to a build-up of pressure drop along the droplet and pushes it through the front barrier. This, in turn, blocks the outlet of the bypasses, and causes a complete release of the merged droplet from the trap (Fig. 3).
Importantly, as the merged droplet contains different solutions at its front and rear (and not side by side), the flow even in a straight channel mixes the two liquids rapidly. This provides for the efficient homogenization of liquids, and the extent of homogenization is controlled more by the distance travelled by the droplet than by the time elapsed from the event of merging. This feature is in line with the idea of encoding the processes into the geometry of the microfluidic device and leaving flexibility on the execution of the flow protocol (e.g. at only loosely controlled speed of flow and timing of events).
The merging trap shown in Fig. 3 has intrusions both at its inlet and outlet. This provides for bi-directional symmetry: the trap will perform the same function irrespective of the direction of flow. It is also possible to merge droplets with intrusions located only at one terminus of the trap. In such a case, upon reversal of flow, the module will be inactive for long droplets and for short ones will act as delay trap.
Fig. 4 Lock&shift trap. a) Scheme of the trap b) Micrographs (1 through 6) illustrate the locking of a droplet (1), the approach of a subsequent droplet (2), blocking of the inlets to the bypasses by the subsequent drop (3) causing the release of the first drop (4), and approach of the second drop to the locked position (5 and 6). The solid lines (arrows) depict schematically the flow of the continuous liquid. The width of the straight segments of the channel is 400 μm. |
The main difference between the metering trap and the lock&shift trap is that in the latter the bypasses are connected with the main channel only at the entrance and at the exit of the trap, while being separated from the main channel along the length of the trap. The lock&shift module can be design with two different types of the obstacle at the end of the trap: one (shown in Fig. 4) with a barrier similar to the one used in the metering trap or second, with two side intrusions, as in the merging trap (See Fig. S2, ESI† and Fig. 5).
The trap locks short droplets: when a droplet flows into the trap, it is pushed towards the barrier and is stopped there by the increased back-acting Laplace pressure. The continuous liquid bypasses the droplet through the parallel channels, decreasing the pressure drop along the droplet. A similar functionality has been shown before by Zagnoni and Cooper,16 the lock&shift trap differs from the shift register unit in that i) it traps a single droplet and releases it upon approach of a consecutive drop, and ii) consecutive droplets never touch each other. We will demonstrate the use of both of these distinct features below.
Fig. 5. shows an illustrative system that uses the metering, delay and lock&shift traps to transform an input of a sample and of a stream of buffer into an array of droplets presenting serial dilutions of the input solution. The device consists of three main parts (see Fig. 5a): (1) the part that transforms a continuous stream of the buffer into 10 precisely metered drops locked in the metering traps. Between the metering traps we placed the delay traps to increase the time of passage, (2) a metering trap for trapping the sample and later to release dilutions and (3) an array of 10 lock & shift traps to array the dilutions.
Fig. 5 Serial dilutor. a) Schematic layout of the device: 1) the array of 10 small metering traps with delay traps (the longer ones) between them, 2) one metering trap used as a dilutor, 3) the array of 10 lock&shift traps; inSam – inlet for the sample; inSol – inlet for the solvent; in1 and in2 – inlets of the continuous phase liquid; out1, out2, outSam – outlets. b)–d) Schematic diagrams of consecutive operations on the device: b) flow of continuous phase between in1 and out1 is switched on, meanwhile a portion of solvent is injected through inSol which leads to the generation of droplets of solvent in 10 metering traps; c) only flow of continuous phase from in1 to outSam is switched on, meanwhile an amount of sample is injected through inSam which results in generation of a droplet of sample in the trap (2). e)–g) Snapshots of the working device: e) device with prepared droplets of solvent (10 small blue droplets) and one droplet of the sample (the large red droplet in the dilutor (2)); f) working device; g) final static array of droplets with varying concentrations. The width of the straight segments of the channel is 400 μm. |
The execution of the protocol comprises three steps illustrated in Fig. 5b–d. First we flow the continuous liquid from inlet in1 to outlet out1 together with an amount of solvent from inlet inSol. The continuous phase pushes a portion of the solvent through the array of metering traps leaving precisely metered aliquots of volume V in them (Fig. 5e). Then we flow the continuous liquid from inlet in1 to outlet outSam and introduce a portion of sample from inSam to meter and lock a precisely metered volume of sample in the metering trap. At this stage all required droplets are metered. In the third step the flow of the continuous liquid from inlet in2 to outlet out2 pushes the drops of buffer into the metering trap containing the sample, just as in the device operated with active droplet-on-demand control.11 The drops, in sequence, merge with the droplet in the sample trap and release an equal volume of the current concentration. While the content of the droplet homogenizes the released droplet travels to the array of the lock&shift traps. Running the whole sequence of droplets through (Fig. 5f) results in a generation of an array of well positioned, metered droplets presenting an exponential decay of the concentration of the sample (Fig. 5g). See also the supplementary video (ESI† mov1).
Fig. 6 Combination of a metering trap (left) and a merging trap (right), one segment of the DOMINO device. The width of the straight segments of the channel is 400 μm. |
We use the above module in an integrated DOMINO system (Fig. 7) to generate an array of precise aliquots of the diluted sample without any requirement for precise control of the rates of flow of the liquids. All the operations are performed simply by switching the direction of flow through the system.
Fig. 7 DOMINO device. a) Schematic view of the device: (1) metering traps; (2) merging trap; (3) single metering of the double length; inSam – inlet of a sample; inSol – inlet of a solvent; in1 and in2 – inlets of the CP; out1 and out2 – outlets of the CP. b)–d) Snapshots of the working device: b) device with prepared droplets of solvent and sample; c) working device; d)–e) final static array of droplets with descending concentration. f)–h) schematic diagrams of consecutive operations on the device. The scale bars are 1 mm. |
The device is operated in 3 steps shown in Fig. 7f–h. In the first step (Fig. 7f) we introduce the flow of the carrier liquid from inlet in1 to outlet out1 and we inject a sufficiently large (>1.375 μL) aliquot of solvent from inlet inSol (all the other inlets/outlets are closed). The continuous liquid pushes the droplet of solvent through the array of traps leaving precisely metered aliquots of volume V = 275 nL in the metering traps (1). In the second step (Fig. 7g) the flow of the continuous liquid is switched on from inlet in2 to outlet out2. This pushes the drops of solvent from the metering traps to the merging traps where they are locked. During this phase, the sample is introduced from inlet inSam and the flow of the continuous liquid locks the precisely metered volume (2V) of sample (of concentration C0) in the metering trap (3). At this stage all required droplets are metered and locked in place (see Fig. 7b).
In the last, third, step (Fig. 7h) we start the flow from the inlet in1 to the outlet out1. This drives the droplet of the sample through the array of traps (see Fig. 7c). In the first metering trap a drop (of volume V) of sample is trapped, passing the remaining (V) to the merging trap where it coalesces with the drop (V) of solvent. The merged droplet is carried (and simultaneously mixed to a homogeneous concentration C1 = C0·V/(V + V) = C0/2) to the next metering trap. Passing through each subsequent set of a metering and merging trap creates a two-fold dilution locked in the metering trap. As a result, after the whole protocol is completed, the device presents an array of precisely metered dilutions (Fig. 7d–e). Importantly the precise operations of i) metering the liquids, ii) merging them in a predetermined sequence, iii) mixing their content, and iv) locking the resulting sequence of droplets at well defined positions was achieved with the control of only the sequence of directions of flow. The exact timing of flow and its rate did not need to be controlled precisely and can be executed e.g. manually. See also supplementary video mov2 (ESI†).
We also used this system to check the standard deviation of the volume of the droplets locked in the metering traps (for details see ESI†). We measured that at a fixed rate of flow the normalized standard deviation of the trapped volumes does not exceed 3%. In addition, within an approximately 3-fold range of rates of flow (e.g. between 100 and 300 μL h−1 or 300 and 750 μL h−1), the same parameter does not exceed 5%. These results confirm that even a very loose control of the rate of inflow of liquids results in precise execution of the protocols hard-wired into the geometry of the chip. This feature distinguishes trap based devices from classical microfluidics geometries, e.g. T-junctions, where even small fluctuations of the rates of flow lead to significant fluctuations in the size of droplets.17
Here, we demonstrated that it is possible to construct systems that can passively perform multiple hard-wired operations on liquid microdroplets. Depending on their design, the individual modules perform a range of elementary operations on micro-aliquots of liquid, including metering, trapping, merging, delaying and releasing droplets. Importantly, these operations can be executed precisely without the requirement for precise control of the rate of flow or the timing of switching the flow on and off. These functions are achieved by a set of hard-wired geometric modules that perform elementary operations on portions of liquids and that can be integrated into networks designed to perform long sequences of operations.
The modules that we describe take advantage of the large imbalance between the interfacial tension forces and the shear stresses, typical of many microfluidic systems. Within this approach, narrower ranges of functions have been demonstrated before. Most of these reports describe geometries acquired via planar fabrication (i.e. with channels of uniform height). For example, a set of pillars was demonstrated to form a merging trap18–20 or a shift register.16 Niu et al.15 used a pillar trap to form a serial dilutor operated with conventionally formed uniform droplets. Bai et al.21 and Xu et al.22 showed an array of microfluidic traps for clustering of large number of droplets. There are also interesting examples of the use of a sequence of traps that allow for the partial exchange of mass with the flow in the main channel, either with the aid of an electric field23 or passively24 without the fully ‘digital’ control of the exchange of mass between the continuous stream and the droplets. Recently, Dangla et al.25 introduced the interesting concept of capillary anchoring that also uses geometry to co-control the flow field in the device via capillary interactions. Van Steijn et al.26 used bypass around a T-junction droplet generator to produce droplets of fixed volume independently of the flow rates of both phases. The interplay between capillary forces and shear stress was also used to construct microfluidic logical gates.27,28
The modules that we present are simple in their fabrication. All of them can be made within a common fabrication scheme (either via milling, embossing, injection moulding or two-layer lithography) that requires structuring of only one of two plates which constitute the microfluidic device. The single coherent approach that we propose can be used to construct modules for all elementary operations on portions of liquids, and show that these modules can be freely arranged into functional networks. As the modules share common design principles of the use of barriers and bypasses, they can be rationally designed, optimized and expanded for a combinatorial number of functions. For example the metering trap can be made symmetric by the introduction of the barriers on both ends. A metering-merging trap can be made by combining the cutting barrier on one side with the merging barrier on the other, etc.
Most importantly, we show that the modules can be arranged into functional systems. We used the model challenge of generation of a sequence of micro-droplets presenting a serial dilution of a sample to demonstrate the ability to integrate the simple modules and to lift the requirements of external control over volumes of droplets issued into the systems, and of the rate of flow of liquids through the chip. We believe that the platform that we report here opens the way for the construction of a broad range of systems that could be operated in passive manner, potentially requiring only deposition of samples onto the chip and the use of gravity to drive the liquids through the traps. As an extension we also believe that it should be possible to design systems that autonomously switch the direction of flow with the use of capillary valves.
The systems that we presented should be useful in both automated and passive droplet microfluidic systems. For example, individual passive modules can be integrated into automated droplet systems to introduce the ability to dilute in screening experiments, to contain drops with microorganisms, while allowing a portion of the liquid to be replaced periodically to sustain long-term growth, or to lock a droplet in place for long-term microscopic observation while allowing a facile replacement of the microscopic sample without cross-contamination. Ultimately, we also hope that the passive modules and the approach that we demonstrated here will be helpful in building a range of microfluidic devices for point-of-care analyses including colorimetric assays of blood with portions of reagents trapped on the chip and the sample processed, aliquoted and delivered to the reagents. Other applications may be for assays in microbiology and molecular biology including the polymerase chain reaction and immunoassays where a sequence of liquid handling operations must be performed on the sample and reaction mixture.
Footnote |
† Electronic supplementary information (ESI) available: Description of the delay trap; lock&shift trap; verification of the precision of the metering traps; two videos showing the operation of the serial dilutors discussed in sections D and F. See DOI: 10.1039/c3lc50347j |
This journal is © The Royal Society of Chemistry 2013 |