Detection of 6-benzylaminopurineplant growth regulator in bean sprouts using OFRR biosensor and QuEChERS method

Abstract

We identified 6-benzylaminopurine (6-BAP) plant growth regulator using an opto-fluidic ring resonator (OFRR) biosensor and a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. In this study, the OFRR biosensor with a bulk refractive index sensitivity (BRIS) of 14.5 nm per RIU was fabricated through the optimization process of the OFRR capillary and tapered fiber optic cable. The detection limit of the OFRR biosensor using the 6-BAP standard solution and 6-BAP sample solution extracted from bean sprouts by the QuEChERS method is 0.01 mg kg⁻¹. This result is similar to the experimental results of HPLC and LC-MS/MS. To apply the OFRR biosensor and the QuEChERS method on the spot, improvement in the accuracy of the OFRR biosensor and the recovery of the QuEChERS method is needed.