Issue 32, 2008
From the journal:

Dalton Transactions

The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Zênis Novais da Rocha,a   Mario Sérgio Pereira Marchesi,b   Josiane Cristina Molin,b   Claure N. Lunardi,b   Katrina M. Miranda,c   Lusiane Maria Bendhack,b   Peter C. Fordd  and  Roberto Santana da Silva*be  

* Corresponding authors

a Instituto de Química da Universidade Federal da Bahia – Campus Ondina, Salvador, Bahia, Brazil

b Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, Ribeirão Preto, SP, Brazil
E-mail: silva@usp.br

c Chemistry Department, University of Arizona, Tucson, AZ, USA

d Department of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, CA, USA

e Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. dos Bandeirantes 3900, Ribeirão Preto, SP, Brazil

Abstract

The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6)3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy)2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2]+. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L−1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10−5 mol L−1 of cis-[RuII(NO2)L(bpy)2]+ complex.

Graphical abstract: The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

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Article information

DOI
https://doi.org/10.1039/B803441A
Article type
Paper
Submitted
28 Feb 2008
Accepted
12 Jun 2008
First published
07 Jul 2008

Dalton Trans., 2008, 4282-4287

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The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Z. N. da Rocha, M. S. P. Marchesi, J. C. Molin, C. N. Lunardi, K. M. Miranda, L. M. Bendhack, P. C. Ford and R. S. da Silva, Dalton Trans., 2008, 4282 DOI: 10.1039/B803441A

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