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DOI: 10.1039/A809656B (Paper) Reference Section for: Anal. Commun., 1999, 36, 47-50

A water-soluble tetrazolium salt useful for colorimetric cell viability assay

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Hideyuki Tominaga, Munetaka Ishiyama, Fumio Ohseto, Kazumi Sasamoto, Tomoyuki Hamamoto, Keiji Suzuki and Masami Watanabe

Abstract

The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [3H]thymidine uptake method.

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