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Fluorogenic probes for imaging reactive oxygen species

Formed as chemical by‐products of cell metabolism, reactive oxygen species (ROS) are connected to multiple pathologies including age‐related disorders, cancer, and neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. New evidence is however emerging pointing to a more complex yet beneficial role ROS play in physiological processes associated with cell signaling. The highly rich and diverse chemistry of ROS, their ubiquitous nature, and their poorly understood biological impact require the development of new chemical tools, including non‐invasive imaging techniques, to explore and elucidate the intrinsic links between the chemistry and biology of ROS. This review highlights recent advances in the development of fluorogenic probes for real‐time visualization in live cells of the generation, accumulation, and consumption of ROS. We will first discuss a number of different photophysical and photochemical processes that may provide intramolecular switches to control the emission of the probes. We will then show how reactions with various specific ROS switch on their emission. Special focus will be placed on recent advances from our group involving the development of lipophilic fluorogenic probes to monitor the production of lipid peroxyl radicals in the lipid membrane of live cells.

Print publication date: 07 Aug 2013
Copyright year: 2013
Print ISBN: 978-1-84973-580-3
PDF eISBN: 978-1-84973-772-2