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Combining Fluorescence Imaging and Ionic Current Detection in Nanocapillaries

We present here recent data showing simultaneous imaging and ionic current detection of DNA fluorescently labeled with SYTOX Orange. Using a fast Electron Multiplying CCD (EMCCD) camera, we have verified the link between ionic current translocation events and the movement of the DNA into the nanocapillary. Further work will focus on elucidating the nature of so-called ‘folding’ DNA events as well as studies on the balance of electrophoresis and electroosmosis in nanocapillaries.

Print publication date: 07 Sep 2012
Copyright year: 2013
Print ISBN: 978-1-84973-416-5
PDF eISBN: 978-1-84973-527-8
Citation: