Issue 21, 2017

Fabrication of a new physiological macroporous hybrid biomaterial/bioscaffold material based on polyphosphate and collagen by freeze-extraction

Abstract

We describe the fabrication of a new scaffold, an inorganic–organic hybrid biomaterial, consisting of the physiological polymers: the inorganic polymer polyphosphate (polyP), as well as the organic macromolecules collagen and chondroitin sulfate. The polyP polymer is composed of multiple phosphate orthophosphate units linked together by high-energy phosphoanhydride bonds. Chondroitin sulfate has been included due to its hydrogel-forming properties. In the presence of Ca2+ ions, the randomly coiled polyP reorganizes together with collagen and chondroitin sulfate to linear molecules which undergo hardening. This scaffold is deposited as amorphous Ca–polyP nanoparticles (size ≈20–40 nm large) around the collagen fibers. Applying the method of freeze-extraction a stable macroporous 3D hydrogel scaffold with a Young's modulus of ≈0.4 MPa is formed comprising cavities/channels larger than 75 μm. The scaffold exhibits a pronounced capacity for induction of cell proliferation (primary human osteoblasts, phOSB) and differentiation (alkaline phosphatase gene expression). We propose that incorporation of the physiological polymer polyP into the collagen-based scaffold might allow a dynamic opening and resealing and by that facilitates the exchange of nutrients for the growth of cells. This physiological hybrid biomaterial might have the potential to be used in bone and cartilage repair.

Graphical abstract: Fabrication of a new physiological macroporous hybrid biomaterial/bioscaffold material based on polyphosphate and collagen by freeze-extraction

Article information

Article type
Paper
Submitted
28 Jan 2017
Accepted
20 Apr 2017
First published
20 Apr 2017

J. Mater. Chem. B, 2017,5, 3823-3835

Fabrication of a new physiological macroporous hybrid biomaterial/bioscaffold material based on polyphosphate and collagen by freeze-extraction

W. E. G. Müller, M. Neufurth, M. Ackermann, E. Tolba, S. Wang, Q. Feng, H. C. Schröder and X. Wang, J. Mater. Chem. B, 2017, 5, 3823 DOI: 10.1039/C7TB00306D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements