A G-quadruplex-based, label-free, switch-on luminescent detection assay for Ag+ ions based on the exonuclease III-mediated digestion of C–Ag+–C DNA

Modi Wang; Ka-Ho Leung; Sheng Lin; Daniel Shiu-Hin Chan; Chung-Hang Leung; Dik-Lung Ma

doi:10.1039/C4TB01140F

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A G-quadruplex-based, label-free, switch-on luminescent detection assay for Ag⁺ ions based on the exonuclease III-mediated digestion of C–Ag⁺–C DNA†

Modi Wang,‡^a Ka-Ho Leung,‡^a Sheng Lin,^a Daniel Shiu-Hin Chan,^a Chung-Hang Leung^b and Dik-Lung Ma*^ac

Author affiliations

* Corresponding authors

^a Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
E-mail: edmondma@hkbu.edu.hk

^b State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China

^c Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, P. R. China

Abstract

We report herein the ability of exonuclease III to cleave C–Ag⁺–C mismatched DNA, which was utilised for the construction of an exonuclease III-assisted, label-free, switch-on luminescent platform for Ag⁺ ions using a novel G-quadruplex-selective iridium(III) complex.

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Article information

DOI: https://doi.org/10.1039/C4TB01140F
Article type: Communication
Submitted: 11 Jul 2014
Accepted: 08 Aug 2014
First published: 11 Aug 2014

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J. Mater. Chem. B, 2014,2, 6467-6471

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A G-quadruplex-based, label-free, switch-on luminescent detection assay for Ag⁺ ions based on the exonuclease III-mediated digestion of C–Ag⁺–C DNA

M. Wang, K. Leung, S. Lin, D. S. Chan, C. Leung and D. Ma, J. Mater. Chem. B, 2014, 2, 6467 DOI: 10.1039/C4TB01140F

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Journal of Materials Chemistry B