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Issue 6, 2013
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Nickel chelating functionalization of graphene composite for metal affinity membrane isolation of lysozyme

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Abstract

A novel functionalized graphene-based composite is prepared by successive modification of graphene oxide (GO) with epichlorohydrin (ECH), iminodiacetic acid (IDA) and 1-phenylboronic acid (1-PBA) through covalent functionalization and then chelating with nickel ions. Characterizations by FT-IR, XRD, SEM, TGA and ICP-MS demonstrate the successful modification of the graphene surface, resulting in a GO–PBA–IDA–Ni composite with a Ni2+ immobilization amount of 3.01 × 10−3 mol g−1. The composite shows favorable selectivity for the adsorption of lysozyme (Lys). In practice, a homogeneous GO–PBA–IDA–Ni film with a thickness of ca. 1.0 μm is fabricated by filtering the composite through a cellulose membrane with a pore aperture of 1.2 μm. The GO–PBA–IDA–Ni film is subsequently fastened into a rounded membrane cartridge and incorporated into a sequential injection system for on-line selective isolation of Lys. An adsorption efficiency of ca. 96% is achieved for 25 μg ml−1 Lys in 500 μl of sample solution at a loading rate of 5 μl s−1. Metal affinity and electrostatic interactions are the main driving forces for governing the protein adsorption behaviors. The retained Lys is readily recovered by a borate buffer (pH 10) containing 1.0 mol l−1 NaCl and 20 mmol l−1 imidazole, giving rise to a recovery of ca. 90%. The practical applicability of the composites is demonstrated by selective isolation of Lys from chicken egg white, and SDS-PAGE assay indicates that Lys is selectively isolated with high purity from complex matrices.

Graphical abstract: Nickel chelating functionalization of graphene composite for metal affinity membrane isolation of lysozyme

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Publication details

The article was received on 31 Oct 2012, accepted on 29 Nov 2012 and first published on 30 Nov 2012


Article type: Paper
DOI: 10.1039/C2TB00334A
Citation: J. Mater. Chem. B, 2013,1, 810-818
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    Nickel chelating functionalization of graphene composite for metal affinity membrane isolation of lysozyme

    J. Liu, T. Yang, S. Chen, X. Chen and J. Wang, J. Mater. Chem. B, 2013, 1, 810
    DOI: 10.1039/C2TB00334A

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