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Issue 19, 2017
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The role of optical projection in the analysis of membrane fluctuations

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Abstract

The spectral analysis of thermal fluctuations, or flickering, is a simple and non-invasive method widely used to determine the mechanical properties of artificial and biological lipid membranes. In its most common implementation, the position of the edge of a cell or vesicle is tracked from optical microscopy videos. However, a systematic disagreement with X-ray scattering and micromechanical manipulation data has brought into question the validity of the method. We present an improved analysis protocol that resolves these discrepancies by accounting for the finite vertical resolution of the optics used to image fluctuations.

Graphical abstract: The role of optical projection in the analysis of membrane fluctuations

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Publication details

The article was received on 16 Jan 2017, accepted on 25 Apr 2017 and first published on 25 Apr 2017


Article type: Communication
DOI: 10.1039/C7SM00108H
Citation: Soft Matter, 2017,13, 3480-3483
  • Open access: Creative Commons BY license
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    The role of optical projection in the analysis of membrane fluctuations

    S. A. Rautu, D. Orsi, L. Di Michele, G. Rowlands, P. Cicuta and M. S. Turner, Soft Matter, 2017, 13, 3480
    DOI: 10.1039/C7SM00108H

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