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Issue 37, 2016
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Melting transition in lipid vesicles functionalised by mobile DNA linkers

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Abstract

We study phase behaviour of lipid-bilayer vesicles functionalised by ligand–receptor complexes made of synthetic DNA by introducing a modelling framework and a dedicated experimental platform. In particular, we perform Monte Carlo simulations that combine a coarse grained description of the lipid bilayer with state of art analytical models for multivalent ligand–receptor interactions. Using density of state calculations, we derive the partition function in pairs of vesicles and compute the number of ligand–receptor bonds as a function of temperature. Numerical results are compared to microscopy and fluorimetry experiments on large unilamellar vesicles decorated by DNA linkers carrying complementary overhangs. We find that vesicle aggregation is suppressed when the total number of linkers falls below a threshold value. Within the model proposed here, this is due to the higher configurational costs required to form inter-vesicle bridges as compared to intra-vesicle loops, which are in turn related to membrane deformability. Our findings and our numerical/experimental methodologies are applicable to the rational design of liposomes used as functional materials and drug delivery applications, as well as to study inter-membrane interactions in living systems, such as cell adhesion.

Graphical abstract: Melting transition in lipid vesicles functionalised by mobile DNA linkers

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Publication details

The article was received on 01 Jul 2016, accepted on 19 Aug 2016 and first published on 19 Aug 2016


Article type: Paper
DOI: 10.1039/C6SM01515H
Citation: Soft Matter, 2016,12, 7804-7817
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    Melting transition in lipid vesicles functionalised by mobile DNA linkers

    S. J. Bachmann, J. Kotar, L. Parolini, A. Šarić, P. Cicuta, L. Di Michele and B. M. Mognetti, Soft Matter, 2016, 12, 7804
    DOI: 10.1039/C6SM01515H

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