Grafted biomembranes containing membrane proteins – the case of the leucine transporter

Vivien Jagalski; Robert D. Barker; Mikkel B. Thygesen; Kamil Gotfryd; Mie B. Krüger; Lei Shi; Selma Maric; Nicolas Bovet; Martine Moulin; Michael Haertlein; Thomas Günther Pomorski; Claus J. Loland; Marité Cárdenas

doi:10.1039/C5SM01490E

Grafted biomembranes containing membrane proteins – the case of the leucine transporter†

Vivien Jagalski,‡^ab Robert D. Barker,‡*^c Mikkel B. Thygesen,^b Kamil Gotfryd,§^d Mie B. Krüger,^d Lei Shi,^e Selma Maric,¶^f Nicolas Bovet,^a Martine Moulin,^c Michael Haertlein,^c Thomas Günther Pomorski,^f Claus J. Loland^d and Marité Cárdenas*^ag

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* Corresponding authors

^a Department of Chemistry and Nano-Science Center, University of Copenhagen, Universitetsparken 5, Copenhagen, Denmark
E-mail: cardenas@nano.ku.dk

^b Department of Chemistry and Nano-Science Center, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

^c Institute Laue Langevin, 71 avenue des Martyrs, 38042 Grenoble Cedex 9, France
E-mail: barker@ill.eu

^d Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

^e Department of Physiology and Biophysics and Institute for Computational Biomedicine, Weill Cornell Medical College, New York, USA

^f Centre for Membrane Pumps in Cells and Disease (PUMPKIN), Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

^g Malmo University, Biofilm – Research Center for Biointerfaces and Department of Biomedical Science, Health & Society, 20506 Malmoe, Sweden
E-mail: marite.cardenas@mah.se

Abstract

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent–lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

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Grafted biomembranes containing membrane proteins – the case of the leucine transporter†

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