Proteo-lipobeads for the oriented encapsulation of membrane proteins

Pinar Frank; Bernhard Siebenhofer; Theresa Hanzer; Andreas F. Geiss; Florian Schadauer; Ciril Reiner-Rozman; Bill Durham; Leslie M. Loew; Bernd Ludwig; Oliver-M. H. Richter; Christoph Nowak; Renate L. C. Naumann

doi:10.1039/C4SM02646B

Proteo-lipobeads for the oriented encapsulation of membrane proteins†

Pinar Frank,‡^a Bernhard Siebenhofer,‡^a Theresa Hanzer,‡^a Andreas F. Geiss,^a Florian Schadauer,^a Ciril Reiner-Rozman,^a Bill Durham,^b Leslie M. Loew,^c Bernd Ludwig,^d Oliver-M. H. Richter,^d Christoph Nowak^e and Renate L. C. Naumann*^a

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^a Biosensor Technologies, Austrian Institute of Technology GmbH, AIT, Donau-City Str. 1, 1220 Vienna, Austria
E-mail: Renate.Naumann@ait.ac.at

^b Department of Chemistry, University of Arkansas, Fayetteville, AR 72701, USA

^c R. D. Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, Connecticut 06030, USA

^d Biocenter, Goethe University, Inst. of Biochemistry, Max-von-Laue-Str. 9, Frankfurt, Germany

^e Center of Electrochemical Surface Technology, CEST, Viktor-Kaplan-Straße 2, 2700 Wiener Neustadt, Austria

Abstract

As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

Soft Matter

Proteo-lipobeads for the oriented encapsulation of membrane proteins†

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