Issue 23, 2014
From the journal:

Soft Matter

Influence of sorbitol on protein crowding in solution and freeze-concentrated phases†

S. Khodadadi,*a   N. J. Clark,bc   A. McAuley,c   V. Cristiglio,d   J. E. Curtis,e   E. Y. Shalaevf  and  S. Krueger*g  

* Corresponding authors

a Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands
E-mail: s.khodadadi@tudelft.nl

b Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD, USA
E-mail: nicholas.clark@nist.gov

c Amgen, Inc, Thousand Oaks, CA, USA
E-mail: arnoldm@amgen.com

d Institut Laue-Langevin, Grenoble, France
E-mail: cristiglio@ill.fr

e Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD, USA
E-mail: joseph.curtis@nist.gov

f Allergan, Inc., Irvine, CA, USA
E-mail: Shalaev_Evgenyi@allergan.com

g Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD, USA
E-mail: susan.krueger@nist.gov

Abstract

Small-angle neutron scattering was employed to study protein crowding under freezing conditions that mimic those used in pharmaceutical processing. The results demonstrate that, although there is an increase in heterogeneity as the temperature is reduced, sorbitol reduces protein crowding in both solution and freeze-concentrated phases, thus protecting the protein from forming oligomers or irreversible aggregates.

Graphical abstract: Influence of sorbitol on protein crowding in solution and freeze-concentrated phases

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Article information

DOI
https://doi.org/10.1039/C4SM00600C
Article type
Communication
Submitted
19 Mar 2014
Accepted
31 Mar 2014
First published
02 Apr 2014

Soft Matter, 2014,10, 4056-4060

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Influence of sorbitol on protein crowding in solution and freeze-concentrated phases

S. Khodadadi, N. J. Clark, A. McAuley, V. Cristiglio, J. E. Curtis, E. Y. Shalaev and S. Krueger, Soft Matter, 2014, 10, 4056 DOI: 10.1039/C4SM00600C

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