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Issue 32, 2012
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Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection

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Abstract

X-Ray and neutron reflectivity measurements on systems composed of a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayer and transcription-activating-factor derived peptides (TDPs) have allowed us to determine the mechanism of membrane translocation. By monitoring the structural changes of the bilayers caused by the binding of TDPs while systemically varying temperature and TDP concentration, our results revealed the detailed molecular structures of the stepwise interactions that occurred during the translocation of TDP across the lipid bilayers. While little indication of membrane perturbation was observed at low TDP concentrations, we found that the TDP movement across the membrane induced defect formations in the membrane at higher TDP concentrations.

Graphical abstract: Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection

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Publication details

The article was received on 19 Apr 2012, accepted on 12 Jun 2012 and first published on 09 Jul 2012


Article type: Communication
DOI: 10.1039/C2SM25913C
Citation: Soft Matter, 2012,8, 8294-8297
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    Insertion mechanism of cell-penetrating peptides into supported phospholipid membranes revealed by X-ray and neutron reflection

    D. Choi, J. H. Moon, H. Kim, B. J. Sung, M. W. Kim, G. Y. Tae, S. K. Satija, B. Akgun, C.-J. Yu, H. W. Lee, D. R. Lee, J. M. Henderson, J. W. Kwong, K. L. Lam, K. Y. C. Lee and K. Shin, Soft Matter, 2012, 8, 8294
    DOI: 10.1039/C2SM25913C

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