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Issue 8, 2012
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Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

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Abstract

Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5–35.1 °C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of “gold-standard” PEI (25 kDa).

Graphical abstract: Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

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Publication details

The article was received on 01 Nov 2011, accepted on 12 Dec 2011 and first published on 19 Jan 2012


Article type: Paper
DOI: 10.1039/C2SM07083A
Citation: Soft Matter, 2012,8, 2518-2526
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    Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

    H. Bao, Y. Ping, Y. Pan, L. Li, J. Li and L. H. Gan, Soft Matter, 2012, 8, 2518
    DOI: 10.1039/C2SM07083A

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