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Issue 10, 2012
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DNA encapsulation via nanotemplates from cationic block copolymer micelles

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Abstract

We report on a method for the encapsulation of DNA into polymeric nanocapsules. The encapsulation procedure involves three steps: formation of polyplexes between DNA and cationic polymeric micelles; coating of polyplexes with a cross-linked shell; dissociation of polyplexes. In the first step copolymer micelles were obtained by self-assembly of an amphiphilic polystyrene-b-poly(quaternized 2-vinylpyridine) (PS-b-PQ2VP) block copolymer in aqueous solution. Nanosized polyplexes (Dh = 190 nm and ζ = −21 mV) were formed upon the addition of DNA to the micellar solution at a phosphate/amine group ratio of 3 : 1. In the second step the complexes were coated with a cross-linked shell formed by seeded radical polymerization of N-isopropylacrylamide which resulted in a slight shift of ζ potential of the particles to a less negative value. The dissociation of polyplexes and removal of PS-b-PQ2VP were achieved by addition of salt and solvent exchange. Following rehydration viadialysis against water, polymeric nanocapsules with entrapped DNA were obtained. The nanocapsules were visualized by transmission electron microscopy; they exhibited smaller dimensions compared to the initial polyplexes and ζ potential very close to that of the pristine DNA.

Graphical abstract: DNA encapsulation via nanotemplates from cationic block copolymer micelles

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Publication details

The article was received on 24 Oct 2011, accepted on 05 Jan 2012, published on 30 Jan 2012 and first published online on 30 Jan 2012


Article type: Paper
DOI: 10.1039/C2SM07029D
Citation: Soft Matter, 2012,8, 2884-2889
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    DNA encapsulation via nanotemplates from cationic block copolymer micelles

    E. Haladjova, S. Rangelov, Ch. B. Tsvetanov and S. Pispas, Soft Matter, 2012, 8, 2884
    DOI: 10.1039/C2SM07029D

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