A mild and versatile approach for DNA encapsulation

Abstract

The complex between polycation-containing thermally sensitive block copolymer and DNA (polyplex) was stabilized utilizing a seeded radical copolymerization in aqueous media under mild conditions. Thus, the DNA molecule was encapsulated into the core of a stable nanoparticle with a cross-linked polymer shell and size of ∼100 nm. The versatility of the proposed approach was demonstrated through the introduction of targeting and biodegradable functions during the polymer shell formation providing an opportunity to construct gene carriers that are able to satisfy the conflicting requirements of adequate DNA protection and efficient release into the target cell.