Issue 18, 2011

PAMAM6 dendrimers and DNA: pH dependent “beads-on-a-string” behavior revealed by small angle X-ray scattering

Abstract

DNA interactions with polycations are not only important for our understanding of chromatin compaction but also for characterizing DNA-binding proteins involved in transcription, replication and repair. DNA is known to form several types of liquid-crystalline phases depending, among other factors, on the polycation structure and charge density. Theoretical studies and simulations have predicted the wrapping of DNA around spherical positively charged polycations. As a potential mimic of the histone octamer or other DNA wrapping proteins, poly(amido amine) generation 6 (PAMAM6) dendrimers have been chosen for our study. The self-assembly of DNA induced by PAMAM6 has been investigated using small angle X-ray scattering (SAXS) in order to reveal the assemblies' structure dependence on the pH of the environment and dendrimer concentrations. We demonstrate that at pH 8.5 dense phases are formed and characterized by a 2D-columnar hexagonal lattice which is transformed into a 3D hexagonal lattice with increasing dendrimer concentration (charge ratio N/P). Moreover, a systematic analysis of the scattering data collected from diluted samples at pH 8.5 and 5.5 has led us to propose a wrapping scenario of DNA around PAMAM6 at pH 5.5.

Graphical abstract: PAMAM6 dendrimers and DNA: pH dependent “beads-on-a-string” behavior revealed by small angle X-ray scattering

Article information

Article type
Paper
Submitted
08 Apr 2011
Accepted
29 Jun 2011
First published
04 Aug 2011

Soft Matter, 2011,7, 8343-8351

PAMAM6 dendrimers and DNA: pH dependent “beads-on-a-string” behavior revealed by small angle X-ray scattering

R. Dootz, A. C. Toma and T. Pfohl, Soft Matter, 2011, 7, 8343 DOI: 10.1039/C1SM05632H

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