Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 20, 2010
Previous Article Next Article

Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI

Author affiliations

Abstract

Two recurring problems with stem/neural progenitor cell (NPC) transplantation therapies for spinal cord injury (SCI) are poor cell survival and uncontrolled cell differentiation. The current study evaluated the viability and differentiation of embryonic stem cell-derived neural progenitor cells (ESNPCs) transplanted within fibrin scaffolds containing growth factors (GFs) and a heparin-binding delivery system (HBDS) to enhance cell survival and direct differentiation into neurons. Mouse ESNPCs were generated from mouse embryonic stem cells (ESCs) using a 4−/4+ retinoic acid (RA) induction protocol that resulted in a population of cells that was 70% nestin positive NPCs. The ESNPCs were transplanted directly into a rat subacute dorsal hemisection lesion SCI model. ESNPCs were either encapsulated in a fibrin scaffold; encapsulated in fibrin containing the HBDS, neurotrophin-3 (NT-3) and platelet derived growth factor (PDGF-AA); or encapsulated in fibrin scaffolds with NT-3 and PDGF-AA without the HBDS. We report that the combination of GFs and fibrin scaffold (without HBDS) enhanced the total number of ESNPCs present in the treated spinal cords and increased the number of ESNPC-derived NeuN positive neurons 8 weeks after transplantation. All experimental groups treated with ESNPCs exhibited an increase in behavioral function 4 weeks after transplantation. In a subset of animals, the ESNPCs over-proliferated as evidenced by SSEA-1 positive/Ki67 positive ESCs found at 4 and 8 weeks. These results demonstrate the potential of tissue-engineered fibrin scaffolds to enhance the survival of NPCs and highlight the need to purify cell populations used in therapies for SCI.

Graphical abstract: Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 29 Mar 2010, accepted on 22 Jul 2010 and first published on 23 Aug 2010


Article type: Paper
DOI: 10.1039/C0SM00173B
Citation: Soft Matter, 2010,6, 5127-5137
  •   Request permissions

    Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI

    P. J. Johnson, A. Tatara, D. A. McCreedy, A. Shiu and S. E. Sakiyama-Elbert, Soft Matter, 2010, 6, 5127
    DOI: 10.1039/C0SM00173B

Search articles by author