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Issue 12, 2010
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Protein decorated membranes by specific molecular interactions

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Here we characterize new metal-functionalized amphiphilic diblock copolymers, developed for both surface and solution molecular recognition applications. Polybutadiene-block-poly(ethylene oxide) copolymers functionalized with nitrilotriacetic acid and tris(nitrilotriacetic acid) were complexed with nickel(II) to obtain coordination sites for oligohistidine residues of model proteins. Mixtures of functionalized polymers with the respective non-functionalized block copolymers self-assemble in aqueous solution into vesicular structures with a controlled density of the metal end-groups on their surface. In solution, binding of His6-tagged green fluorescent protein (EGFP) and red fluorescent protein (RFP) to the vesicle surface was quantified by fluorescence correlation spectroscopy. Small-angle X-ray scattering indicates an increase of the membrane thickness by 2–3 nm upon protein binding. Block copolymer monolayers at the air–water interface and on solid support served as a model system to characterize the protein-decorated membranes by Brewster angle microscopy and AFM. High resolution AFM of solid-supported, hydrated monolayers indicates that the proteins form densely packed and partially ordered arrays with the cylindrically shaped EGFP molecules lying flat on the surface of the films.

Graphical abstract: Protein decorated membranes by specific molecular interactions

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The article was received on 10 Feb 2010, accepted on 15 Apr 2010 and first published on 18 May 2010

Article type: Paper
DOI: 10.1039/C002838J
Citation: Soft Matter, 2010,6, 2815-2824
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    Protein decorated membranes by specific molecular interactions

    R. Nehring, C. G. Palivan, S. Moreno-Flores, A. Mantion, P. Tanner, J. L. Toca-Herrera, A. Thünemann and W. Meier, Soft Matter, 2010, 6, 2815
    DOI: 10.1039/C002838J

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