Jump to main content
Jump to site search

Issue 9, 2016
Previous Article Next Article

The rational design of specific SOD1 inhibitors via copper coordination and their application in ROS signaling research

Author affiliations

Abstract

Efficient methods for the regulation of intracellular O2˙ and H2O2 levels, without altering intracellular processes, are urgently required for the rapidly growing interest in ROS signaling, as ROS signaling has been confirmed to be involved in a series of basic cellular processes including proliferation, differentiation, growth and migration. Intracellular H2O2 is formed mainly via the catalytic dismutation of O2˙ by SODs including SOD1, SOD2 and SOD3. Thus, the intracellular levels of O2˙ and H2O2 can directly be controlled through regulating SOD1 activity. Here, based on the active site structure and catalytic mechanism of SOD1, we developed a new type of efficient and specific SOD1 inhibitors which can directly change the intracellular levels of H2O2 and O2˙. These inhibitors inactivate intracellular SOD1 via localization into the SOD1 active site, thereby coordinating to the Cu2+ in the active site of SOD1, blocking the access of O2˙ to Cu2+, and breaking the Cu2+/Cu+ catalytic cycle essential for O2˙ dismutation. The reduced ERK1/2 phosphorylation induced by the specific SOD1 inactivation-mediated decrease of intracellular H2O2 levels reveals the potential of these specific SOD1 inhibitors in understanding and regulating ROS signaling. Furthermore, these specific SOD1 inhibitors also lead to selectively elevated cancer cell apoptosis, indicating that these kinds of SOD1 inhibitors might be candidates for lead compounds for cancer treatment.

Graphical abstract: The rational design of specific SOD1 inhibitors via copper coordination and their application in ROS signaling research

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Mar 2016, accepted on 15 Jun 2016 and first published on 16 Jun 2016


Article type: Edge Article
DOI: 10.1039/C6SC01272H
Citation: Chem. Sci., 2016,7, 6251-6262
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    The rational design of specific SOD1 inhibitors via copper coordination and their application in ROS signaling research

    X. Dong, Z. Zhang, J. Zhao, J. Lei, Y. Chen, X. Li, H. Chen, J. Tian, D. Zhang, C. Liu and C. Liu, Chem. Sci., 2016, 7, 6251
    DOI: 10.1039/C6SC01272H

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements