Issue 5, 2016

Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

Abstract

DNA three-way junctions (3WJs) are essential structural motifs for DNA nanoarchitectures and DNA-based materials. We report herein a metal-responsive structural transformation between DNA duplexes and 3WJs using artificial oligonucleotides modified with a 2,2′-bipyridine (bpy) ligand. A mixture of bpy-modified DNA strands and natural complementary strands were self-assembled exclusively into duplexes without any transition metal ions, while they formed 3WJs in the presence of NiII ions. This transformation was induced by the formation of an interstrand NiII(bpy)3 complex, which served as a template for the 3WJ assembly. Altering the amount and identity of the metal ion regulated the 3WJ induction efficiency. Removal of the metal using EDTA quantitatively regenerated the duplexes. The metal-dependent structural conversion shown here has many potential applications in the development of stimuli-responsive DNA materials.

Graphical abstract: Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

Supplementary files

Article information

Article type
Edge Article
Submitted
26 Jan 2016
Accepted
15 Feb 2016
First published
16 Feb 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 3006-3010

Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

Y. Takezawa, S. Yoneda, J. H. A. Duprey, T. Nakama and M. Shionoya, Chem. Sci., 2016, 7, 3006 DOI: 10.1039/C6SC00383D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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