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Issue 7, 2016
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Delivering the cell-impermeable DNA ‘light-switching’ Ru(II) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method

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Abstract

The dipyridophenazine (dppz) based ruthenium polypyridyl complexes are known as molecular ‘light-switches’ for DNA. This property is poised to serve in diagnostic and therapeutic applications, but the poor cellular uptake restricts their use in live cells. Herein, we show that the cellular uptake, and more interestingly and surprisingly, the nuclear uptake of cell-impermeable Ru(II)–polypyridyl cationic complexes such as [Ru(bpy)2(dppz)]2+ were remarkably enhanced by three structurally unrelated biochemical agents (pentachlorophenol, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and tolfenamic acid), by forming lipophilic and relatively stable ion-pair complexes, via a passive diffusion mechanism. Enantioselective imaging of live-cell nuclear DNA was observed between the two chiral forms of Ru(II) complexes. This represents the first report of an unprecedented new method for delivering the DNA ‘light-switching’ Ru(II) complexes into the nucleus of living cells via ion-pairing, which could serve as a promising general live-cell delivery method for other potentially bio-medically important but cell-impermeable metal complexes.

Graphical abstract: Delivering the cell-impermeable DNA ‘light-switching’ Ru(ii) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method

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Publication details

The article was received on 06 Oct 2015, accepted on 20 Jan 2016 and first published on 05 Apr 2016


Article type: Edge Article
DOI: 10.1039/C5SC03796D
Citation: Chem. Sci., 2016,7, 4016-4023
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    Delivering the cell-impermeable DNA ‘light-switching’ Ru(II) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method

    B. Zhu, X. Chao, C. Huang and Y. Li, Chem. Sci., 2016, 7, 4016
    DOI: 10.1039/C5SC03796D

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