Jump to main content
Jump to site search

Issue 1, 2016
Previous Article Next Article

Protein-specific Raman imaging of glycosylation on single cells with zone-controllable SERS effect

Author affiliations

Abstract

A zone-controllable SERS effect is presented for Raman imaging of protein-specific glycosylation on a cell surface using two types of newly designed nanoprobes. The signal probe, prepared using a Raman signal molecule and dibenzocyclooctyne-amine to functionalize a 10 nm Au nanoparticle, exhibits a negligible SERS effect and can recognize and link the azide-tagged glycan via a click reaction. The substrate probe, an aptamer modified 30 or 40 nm Au nanoparticles, can specifically recognize the target protein to create an efficient SERS zone on the target protein. By controlling the size of the substrate probe to match the expression zone of the protein-specific glycan, an efficient SERS signal can be generated. This method has been successfully used for in situ imaging of sialic acids on the target protein EpCAM on an MCF-7 cell surface and for the monitoring of the expression variation of protein-specific glycosylation during drug treatment. The concept of zone control can also be used to measure the distance between glycoproteins on a cell surface. This protocol shows promise in uncovering glycosylation-related biological processes.

Graphical abstract: Protein-specific Raman imaging of glycosylation on single cells with zone-controllable SERS effect

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Sep 2015, accepted on 15 Oct 2015 and first published on 16 Oct 2015


Article type: Edge Article
DOI: 10.1039/C5SC03560K
Author version
available:
Download author version (PDF)
Citation: Chem. Sci., 2016,7, 569-574
  • Open access: Creative Commons BY license
  •   Request permissions

    Protein-specific Raman imaging of glycosylation on single cells with zone-controllable SERS effect

    Y. Chen, L. Ding, W. Song, M. Yang and H. Ju, Chem. Sci., 2016, 7, 569
    DOI: 10.1039/C5SC03560K

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements