Jump to main content
Jump to site search

Issue 12, 2013
Previous Article Next Article

Development of cell-impermeable coelenterazine derivatives

Author affiliations

Abstract

We describe the development of the first cell-membrane impermeable coelenterazine derivative (CoelPhos). CoelPhos was constructed by the alkylation of coelenterazine with a linker containing a terminal anionic phosphonate moiety. The bioluminescence activity of CoelPhos with Gaussia luciferase (GLuc) showed a significantly higher activity in comparison with Renilla luciferase. In imaging studies with living cells, outer membrane bound GLuc was clearly imaged with CoelPhos. On the other hand no signal could be detected with intracellularly localized GLuc, demonstrating the impermeability of this novel coelenterate substrate derivative. CoelPhos has potential utility as a new bioluminogenic tool for the monitoring of dynamic fusion events at the cell–surface interface.

Graphical abstract: Development of cell-impermeable coelenterazine derivatives

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Jul 2013, accepted on 03 Sep 2013 and first published on 03 Sep 2013


Article type: Edge Article
DOI: 10.1039/C3SC51985F
Citation: Chem. Sci., 2013,4, 4395-4400
  • Open access: Creative Commons BY license
  •   Request permissions

    Development of cell-impermeable coelenterazine derivatives

    E. Lindberg, S. Mizukami, K. Ibata, T. Fukano, A. Miyawaki and K. Kikuchi, Chem. Sci., 2013, 4, 4395
    DOI: 10.1039/C3SC51985F

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements