Issue 9, 2013
From the journal:

Chemical Science

A novel approach to the site-selective dual labelling of a proteinvia chemoselective cysteine modification†

Ramiz I. Nathani,a   Paul Moody,a   Vijay Chudasama,a   Mark E. B. Smith,a   Richard J. Fitzmauricea  and  Stephen Caddick*a  

* Corresponding authors

a Department of Chemistry, University College London, 20 Gordon Street, London, UK
E-mail: VPEnterprise@ucl.ac.uk
Fax: +44 (0)20 7679 7463
Tel: +44 (0)20 3108 5071

Abstract

Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.

Graphical abstract: A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

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Article information

DOI
https://doi.org/10.1039/C3SC51333E
Article type
Edge Article
Submitted
13 May 2013
Accepted
17 Jun 2013
First published
18 Jun 2013
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2013,4, 3455-3458

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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

R. I. Nathani, P. Moody, V. Chudasama, M. E. B. Smith, R. J. Fitzmaurice and S. Caddick, Chem. Sci., 2013, 4, 3455 DOI: 10.1039/C3SC51333E

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