Issue 52, 2016, Issue in Progress

Fabrication of magnetite nanoparticle doped reduced graphene oxide grafted polyhydroxyalkanoate nanocomposites for tissue engineering application

Abstract

In tissue engineering, magnetic nanoparticle based polymeric nanocomposites are attractive due to some superior properties that are demonstrated in monitoring the nature of cell proliferation, differentiation and the activation of cell construction in the tissue regeneration phase. Herein, we have developed a non-toxic, antimicrobial, biocompatible and biodegradable magnetic Fe3O4/RGO-g-PHBV composite based porous 3D scaffold. The facile and cost-effective green pathways were chosen to reduce the exfoliated graphite oxide using a new microbial strain, Lysinibacillus fusiformis at room temperature. The reduction of exfoliated graphite oxide and the fabrication of iron nanoparticle embedded Fe3O4/RGO-g-PHBV nanocomposite were confirmed by X-ray powder diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The analysis of the stretching vibrations by Raman spectroscopy indicated that both the graphite oxide and reduced graphene oxide exhibit frequencies at nearly 1560 cm−1 (G-band) and 1307 cm−1 (D-band). Field Emission Scanning Electron Microscopy (FESEM) and high-resolution transmission electron microscopic studies demonstrated the exfoliated nano sheets of the graphite oxide and the uniform distribution of the deposited ferrite nanoparticles. The inclusion of magnetite nanoparticles and reduced graphene oxide in the network of the PHBV matrix revealed the improvement of the mechanical strength of the nanocomposite, in comparison to the pure PHBV copolymer. The magnetic properties measured by vibrating sample magnetometer (VSM) and magnetic imaging resonance (MRI) confirmed the super-paramagnetic behavior of the nanocomposite, evidenced by the saturation magnetization having low coercive field and dark contrast images in the presence of applied magnetic fields. The confocal and scanning electron microscopy analyses demonstrated the excellent fibroblast cell infiltration, adhesion and proliferation into the micro-porous 3D scaffold, indicating the biocompatibility of the Fe3O4/RGO-g-PHBV nanocomposite based supporting biomaterials.

Graphical abstract: Fabrication of magnetite nanoparticle doped reduced graphene oxide grafted polyhydroxyalkanoate nanocomposites for tissue engineering application

Supplementary files

Article information

Article type
Paper
Submitted
03 Feb 2016
Accepted
25 Apr 2016
First published
27 Apr 2016

RSC Adv., 2016,6, 46116-46133

Fabrication of magnetite nanoparticle doped reduced graphene oxide grafted polyhydroxyalkanoate nanocomposites for tissue engineering application

N. Pramanik, J. De, R. K. Basu, T. Rath and P. P. Kundu, RSC Adv., 2016, 6, 46116 DOI: 10.1039/C6RA03233H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements