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Issue 46, 2013
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Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor

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Abstract

A new turn-on Cu2+ fluorescent sensor (CST) having a trehalose moiety, which confers a relatively large solubility in water, has been synthesized. The chemosensor is therefore suitable for studies in aqueous solution. Full potentiometric and UV-vis characterization evidence that at physiological pH CST forms with Cu2+ a species with a 1 : 1 stoichiometry allowing for a straightforward correlation between CST response and copper(II) concentration. The presence of the trehalose unit does not negatively affect the selectivity of CST for Cu2+ over a series of metal ions of interest as proven by fluorescence measurements. The novel chemosensor, tested in differentiated neuroblastoma SH-SY5Y cells, is able to detect Cu2+ in the extracellular region, as well as to track copper transfer processes upon cell stimulation induced by cellular depolarization.

Graphical abstract: Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor

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Publication details

The article was received on 29 Jul 2013, accepted on 09 Oct 2013 and first published on 10 Oct 2013


Article type: Paper
DOI: 10.1039/C3RA43988G
Citation: RSC Adv., 2013,3, 24288-24297
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    Ratiometric fluorescence sensing and cellular imaging of Cu2+ by a new water soluble trehalose-naphthalimide based chemosensor

    G. I. Grasso, S. Gentile, M. L. Giuffrida, C. Satriano, C. Sgarlata, M. Sgarzi, G. Tomaselli, G. Arena and L. Prodi, RSC Adv., 2013, 3, 24288
    DOI: 10.1039/C3RA43988G

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