Issue 37, 2013

Sensitive electrochemical immunoassay of IgG1 based on poly(amido amine) dendrimer-encapsulated CdS quantum dots

Abstract

Herein, we designed a new electrochemical immunoassay protocol for the detection of IgG1, as a model biomolecule, by using poly(amidoamine) dendrimer-encapsulated CdS quantum dots (DEQD) as molecular tags. Initially, monoclonal anti-human IgG1 specific antibody (mAb1) was anchored on a bovine serum albumin-modified glassy carbon electrode preferably selective to capture Fab of IgG1 from the sample. For the determination, monoclonal anti-human IgG1 (Fc specific) antibody (mAb2) was covalently bound onto the as-prepared DEQD based on a typical carbodiimide coupling. With a sandwich-type immunoassay format, the subsequent anodic stripping voltammetric detection of cadmium released under acidic condition from the coupled quantum dots (QD) was conducted in 2-mL pH 2.5 HCl containing 0.01 M KCl. Under optimal conditions, the dynamic concentration range of the developed immunoassay spanned from 0.1 pg mL−1 to 100 ng mL−1 IgG1 with a detection limit (LOD) of 0.06 pg mL−1 toward IgG1. The electrochemical immunoassay showed good reproducibility, selectivity and stability. The analysis of human serum specimens were in good accordance with the results obtained by a referenced enzyme-linked immunosorbent assay (ELISA) method.

Graphical abstract: Sensitive electrochemical immunoassay of IgG1 based on poly(amido amine) dendrimer-encapsulated CdS quantum dots

Article information

Article type
Paper
Submitted
07 Apr 2013
Accepted
04 Jul 2013
First published
09 Jul 2013

RSC Adv., 2013,3, 16410-16415

Sensitive electrochemical immunoassay of IgG1 based on poly(amido amine) dendrimer-encapsulated CdS quantum dots

X. Pei, Z. Xu, J. Zhang, Z. Liu and J. Tian, RSC Adv., 2013, 3, 16410 DOI: 10.1039/C3RA41665H

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