Issue 8, 2011
From the journal:

RSC Advances

Integrated in situ genetic analyzer for microbiology in extreme environments†

Tatsuhiro Fukuba,ab   Akimitsu Miyaji,c   Takuji Okamoto,d   Takatoki Yamamoto,e   Shohei Kanedaf  and  Teruo Fujii*af  

* Corresponding authors

a Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Tokyo, Meguro-ku, Japan

b UT Ocean Alliance, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, Japan

c Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan

d Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan

e Department of Mechanical and Control Engineering, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan

f LIMMS-CNRS/IIS (UMI 2080), 4-6-1 Komaba, Tokyo, Meguro-ku, Japan

Abstract

In this study, a totally integrated in situ analyzer for microbial gene detection has been developed for oceanography applications. A PDMS–glass microfluidic device that is capable of cell lysis, DNA purification, PCR, and optical detection has been utilized as the core element of the in situ analyzer. Microbial genomic DNA is purified and concentrated on glass beads packed in the microfluidic device. PCR is performed in a flow-through manner, and the amplified products are fluorescently detected using optical fibers. The sensitivity of a completed analyzer with deep-sea operation capabilities has been evaluated in a laboratory setting, and the analyzer has been operated in real deep-sea environments. Field evaluations have shown that the amplification of the eubacterial universal 16S rRNA gene and the recovery of the PCR product to the surface are successfully achieved in deep-sea environments.

Graphical abstract: Integrated in situ genetic analyzer for microbiology in extreme environments

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1RA00490E
Article type
Paper
Submitted
20 Jul 2011
Accepted
22 Aug 2011
First published
21 Oct 2011

RSC Adv., 2011,1, 1567-1573

Permissions

Request permissions

Integrated in situ genetic analyzer for microbiology in extreme environments

T. Fukuba, A. Miyaji, T. Okamoto, T. Yamamoto, S. Kaneda and T. Fujii, RSC Adv., 2011, 1, 1567 DOI: 10.1039/C1RA00490E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements