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Issue 14, 2013
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Fluorescence turn-on detection of DNA based on the aggregation-induced emission of conjugated poly(pyridinium salt)s

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Abstract

Herein, a new conjugated poly(pyridinium salt), poly{(4,4′-(1,4-phenylene)bis(2,6-diphenylpyridinium))-co-N-butyl-3,6-carbazolylene ditosylate} (Poly1), exhibits aggregation-induced emission (AIE) characteristics. It is weakly emissive in solution but highly luminescent in the aggregate state. A fluorescence turn-on biosensor for calf thymus DNA (ctDNA) detection and quantification is developed by taking advantage of complexation-induced aggregation of Poly1, which increases the polymer fluorescence in aqueous solution. It was found that the fluorescence intensity of Poly1 in ethanolphosphate buffer solution (2 mM, pH 7.4) (v/v 1 : 1) was increased up to about 2.4 folds when the concentration of ctDNA increased from 0 to 95 μM along with the blue-shift of the emission peak from 604 to 586 nm. In the concentration range from 0 to 60 μM, the ratio of the increase in photoluminescent intensity at 604 nm in the presence of ctDNA (IctDNAI0) to that in the absence of ctDNA (I0) is linearly proportional to the concentration of ctDNA with a correlation coefficient of 0.997. The detection limit was calculated to be of 1.2 μM based on the results of fluorescence titration.

Graphical abstract: Fluorescence turn-on detection of DNA based on the aggregation-induced emission of conjugated poly(pyridinium salt)s

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Publication details

The article was received on 15 Mar 2013, accepted on 09 May 2013 and first published on 09 May 2013


Article type: Paper
DOI: 10.1039/C3PY00350G
Citation: Polym. Chem., 2013,4, 4045-4051
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    Fluorescence turn-on detection of DNA based on the aggregation-induced emission of conjugated poly(pyridinium salt)s

    J. Sun, Y. Lu, L. Wang, D. Cheng, Y. Sun and X. Zeng, Polym. Chem., 2013, 4, 4045
    DOI: 10.1039/C3PY00350G

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