Reversible logic gate modulated by nucleases based on cationic conjugated polymer/DNA assembly

Abstract

Reversible logic gates were designed and prepared by modulating the logic inputs based on the original system combining new cationic polyfluorene PFPB with labelled dsDNA. The NOT, YES, and NOR logic gates were built with T4 ligase and restriction endonucleases such as HaeIII and PvuII as the inputs and the emission intensity of dyes (FAM or TAMRA) as the output. Most importantly, the logic gates YES and NOT can switch back and forth by the addition of HaeIII and T4 ligase. By a similar method, the logic gates NOR and YES also realized this recycling. This study is a promising starting point for constructing reusable systems for biosensors in the multiplex detection of nucleases.