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Issue 10, 2013
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Effects of oral administration of Lonicera caerulea berries on UVB-induced damage in SKH-1 mice. A pilot study

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Abstract

Solar ultraviolet radiation is a major environmental factor that has serious adverse effects on the structure and function of the skin. Although the UVB waveband (295–315 nm) represents only 5–10% of incoming UV light, it is very damaging to the skin. The aim of this study was to investigate the effect of Lonicera caerulea berries on UVB-induced damage to SKH-1 hairless mice. Mice were fed a L. caerulea berry-enriched diet (10%, w/w) for 14 days before a single UVB (1000 mJ cm−2) treatment. Effects on health status, antioxidant enzyme activity and expression, and DNA damage were evaluated. The bioavailability of L. caerulea phenolic components was also assessed. We found that feeding with L. caerulea berries prevented a decrease in catalase activity and stimulated NADPH quinone oxidoreductase-1, heme oxygenase-1, and gamma-glutamylcysteine synthetase catalytic and modulatory subunit expression in UVB exposed mice. Administration of the L. caerulea berry-enriched diet led to an increase in UVB-reduced interleukin-17 levels and a decrease in keratinocyte-derived chemokine protein expression that was enhanced after UVB treatment. Further, L. caerulea berries reduced UVB-induced DNA damage evaluated as number of single strand breaks, cyclobutane–pyrimidine dimer formation and H2AX phosphorylation, a marker of double strand breaks. Taken together, we provide evidence that oral administration of L. caerulea berries to mice affords at least partial protection from the adverse effects of a single UVB exposure via modulation of antioxidant enzyme activity/expression and reduction of DNA damage.

Graphical abstract: Effects of oral administration of Lonicera caerulea berries on UVB-induced damage in SKH-1 mice. A pilot study

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Publication details

The article was received on 19 Apr 2013, accepted on 03 Jul 2013 and first published on 04 Jul 2013


Article type: Paper
DOI: 10.1039/C3PP50120E
Citation: Photochem. Photobiol. Sci., 2013,12, 1830-1840
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    Effects of oral administration of Lonicera caerulea berries on UVB-induced damage in SKH-1 mice. A pilot study

    A. Rajnochová Svobodová, A. Galandáková, I. Palíková, D. Doležal, D. Kylarová, J. Ulrichová and J. Vostálová, Photochem. Photobiol. Sci., 2013, 12, 1830
    DOI: 10.1039/C3PP50120E

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