Issue 6, 2008
From the journal:

Photochemical & Photobiological Sciences

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellularpH measurements†

Takakazu Nakabayashi,a   Hui-Ping Wang,ab   Masataka Kinjoac  and  Nobuhiro Ohta*a  

* Corresponding authors

a Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo, Japan
E-mail: nohta@es.hokudai.ac.jp
Fax: +81 11 706 4970
Tel: +81 11 706 2594

b Department of Physiology, School of Medicine, Zhejiang University, Hangzhou, China

c Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan

Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

Graphical abstract: Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B800391B
Article type
Communication
Submitted
09 Jan 2008
Accepted
19 Mar 2008
First published
01 Apr 2008

Photochem. Photobiol. Sci., 2008,7, 668-670

Permissions

Request permissions

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

T. Nakabayashi, H. Wang, M. Kinjo and N. Ohta, Photochem. Photobiol. Sci., 2008, 7, 668 DOI: 10.1039/B800391B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements