Issue 7, 2018

Ribosomal incorporation of backbone modified amino acids via an editing-deficient aminoacyl-tRNA synthetase

Abstract

The ability to incorporate non-canonical amino acids (ncAA) using translation offers researchers the ability to extend the functionality of proteins and peptides for many applications including synthetic biology, biophysical and structural studies, and discovery of novel ligands. Here we describe the high promiscuity of an editing-deficient valine-tRNA synthetase (ValRS T222P). Using this enzyme, we demonstrate ribosomal translation of 11 ncAAs including those with novel side chains, α,α-disubstitutions, and cyclic β-amino acids.

Graphical abstract: Ribosomal incorporation of backbone modified amino acids via an editing-deficient aminoacyl-tRNA synthetase

Supplementary files

Article information

Article type
Communication
Submitted
28 Nov 2017
Accepted
16 Jan 2018
First published
25 Jan 2018

Org. Biomol. Chem., 2018,16, 1073-1078

Ribosomal incorporation of backbone modified amino acids via an editing-deficient aminoacyl-tRNA synthetase

E. S. Iqbal, K. K. Dods and M. C. T. Hartman, Org. Biomol. Chem., 2018, 16, 1073 DOI: 10.1039/C7OB02931D

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