Issue 24, 2017

Development of 19F-NMR chemical shift detection of DNA B–Z equilibrium using 19F-NMR

Abstract

Various DNA conformational changes are in correlation with biological events. In particular, DNA B–Z equilibrium showed a high correlation with translation and transcription. In this study, we developed a DNA probe containing 5-trifluoromethylcytidine or 5-trifluoromethylthymidine to detect DNA B–Z equilibrium using 19F-NMR. Its probe enabled the quantitative detection of B-, Z-, and ss-DNA based on 19F-NMR chemical shift change.

Graphical abstract: Development of 19F-NMR chemical shift detection of DNA B–Z equilibrium using 19F-NMR

Supplementary files

Article information

Article type
Communication
Submitted
22 Mar 2017
Accepted
20 Apr 2017
First published
20 Apr 2017

Org. Biomol. Chem., 2017,15, 5109-5111

Development of 19F-NMR chemical shift detection of DNA B–Z equilibrium using 19F-NMR

S. Nakamura, H. Yang, C. Hirata, F. Kersaudy and K. Fujimoto, Org. Biomol. Chem., 2017, 15, 5109 DOI: 10.1039/C7OB00706J

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