Issue 27, 2014

A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

Abstract

In the present study a novel imine-hydrazone based fluorescent chemosensor (L1) for efficient and selective sensing of Zn2+ over other biologically important metal ions under physiological conditions is reported. An enhancement in fluorescence emission intensity of the developed probe with a red shift of ∼25 nm was observed for Zn2+, whereas other metal ions failed to reveal any significant change in the emission spectra. Interestingly, the receptor functioned under completely physiological conditions (99.7% HEPES buffer) and has visible light excitability. Sensing of Zn2+ was investigated in detail by absorption spectroscopy, emission spectroscopy, DFT calculation, 1H-NMR titration experiment and ESI-MS experiment. The association constant between L1 and Zn2+ was found to be 5.58 × 105 M−1. The receptor could detect as low as 69 ppb Zn2+. Sensing of Zn2+ is proposed through switch-on of intramolecular charge transfer (ICT) and chelation enhanced fluorescence (CHEF) processes after the introduction of Zn2+ into the free ligand. The developed receptor was non-toxic and rendered intracellular sensing of Zn2+ in HeLa cells through fluorescence imaging studies.

Graphical abstract: A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

Supplementary files

Article information

Article type
Paper
Submitted
27 Mar 2014
Accepted
13 May 2014
First published
13 May 2014

Org. Biomol. Chem., 2014,12, 4975-4982

Author version available

A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

B. K. Datta, D. Thiyagarajan, S. Samanta, A. Ramesh and G. Das, Org. Biomol. Chem., 2014, 12, 4975 DOI: 10.1039/C4OB00653D

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