The base discriminating potential of pyrrolidinyl PNA demonstrated by magnetic FexOy particles

Claudia Stubinitzky; Tirayut Vilaivan; Hans-Achim Wagenknecht

doi:10.1039/C4OB00487F

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The base discriminating potential of pyrrolidinyl PNA demonstrated by magnetic Fe_xO_y particles†

Claudia Stubinitzky,^a Tirayut Vilaivan*^b and Hans-Achim Wagenknecht*^a

Author affiliations

* Corresponding authors

^a Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany
E-mail: Wagenknecht@kit.edu
Fax: +49-721-608-44825
Tel: +49-721-608-47486

^b Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok 10330, Thailand
E-mail: vtirayut@chula.ac.th
Fax: +66-2-2187598
Tel: +66-2-2187627

Abstract

Pyrrolidinyl PNA was immobilized on Fe_xO_y magnetic particles and was able to capture and thereby discriminate single base alterations in DNA counterstrands better than DNA. The selectivities of matched vs. mismatched oligonucleotides measured by the absorption differences were up to 10–12 which are remarkable values for linear probes.

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Article information

DOI: https://doi.org/10.1039/C4OB00487F
Article type: Communication
Submitted: 04 Mar 2014
Accepted: 08 Apr 2014
First published: 08 Apr 2014

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Org. Biomol. Chem., 2014,12, 3586-3589

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The base discriminating potential of pyrrolidinyl PNA demonstrated by magnetic Fe_xO_y particles

C. Stubinitzky, T. Vilaivan and H. Wagenknecht, Org. Biomol. Chem., 2014, 12, 3586 DOI: 10.1039/C4OB00487F

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Organic & Biomolecular Chemistry