Issue 10, 2014

Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores

Abstract

A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins.

Graphical abstract: Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores

Supplementary files

Article information

Article type
Paper
Submitted
02 Oct 2013
Accepted
15 Jan 2014
First published
15 Jan 2014

Org. Biomol. Chem., 2014,12, 1603-1610

Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores

F. Di Maria, I. E. Palamà, M. Baroncini, A. Barbieri, A. Bongini, R. Bizzarri, G. Gigli and G. Barbarella, Org. Biomol. Chem., 2014, 12, 1603 DOI: 10.1039/C3OB41982G

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