Issue 1, 2014

CGmCGCG is a versatile substrate with which to evaluate Tet protein activity

Abstract

Tet family proteins have the ability to convert 5-methylcytosine (mC) to 5-hydroxymethylcytosine, and further to 5-formylcytosine and 5-carboxycytosine. We found that CGmCGCG can be the substrate of Tet protein, and observed iterative oxidation of mC by HPLC analysis. We also demonstrated that Tet protein favours single-stranded DNA over double-stranded DNA.

Graphical abstract: CGmCGCG is a versatile substrate with which to evaluate Tet protein activity

Supplementary files

Article information

Article type
Paper
Submitted
06 Sep 2013
Accepted
08 Oct 2013
First published
09 Oct 2013

Org. Biomol. Chem., 2014,12, 104-107

CGmCGCG is a versatile substrate with which to evaluate Tet protein activity

S. Kizaki and H. Sugiyama, Org. Biomol. Chem., 2014, 12, 104 DOI: 10.1039/C3OB41823E

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