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Issue 48, 2012
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Highly ordered pyrene π-stacks on an RNA duplex display static excimer fluorescence

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Abstract

The binding and fluorescence properties of complementary RNA sequences attached to different numbers of pyrenes via one carbon linker at the 2′-O-positions have been investigated. Upon hybridization of the pyrene-modified RNA sequences, the modified RNA duplexes with normal thermal stability are formed, and the pyrene arrays are assembled in an inter-strand manner. Because hypochromic effects in the pyrene absorption band and the exciton coupled circular dichroism signals were observed for the pyrene assemblies, the formation of the pyrene array occurs via a π-stacking interaction between the pyrene rings. The pyrene assemblies exhibit strong excimer fluorescence that is characterized by a broad and structureless excitation spectrum. Hence, the excimer is a static excimer due to the direct excitation of the associated pyrenes in the ground state. Based on several spectroscopies, it is revealed that the spatial configuration of the pyrenes in the association is more regulated by the increase in the attached pyrene.

Graphical abstract: Highly ordered pyrene π-stacks on an RNA duplex display static excimer fluorescence

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Publication details

The article was received on 09 Sep 2012, accepted on 24 Oct 2012 and first published on 25 Oct 2012


Article type: Paper
DOI: 10.1039/C2OB26773J
Citation: Org. Biomol. Chem., 2012,10, 9620-9626
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    Highly ordered pyrene π-stacks on an RNA duplex display static excimer fluorescence

    M. Nakamura, M. Fukuda, T. Takada and K. Yamana, Org. Biomol. Chem., 2012, 10, 9620
    DOI: 10.1039/C2OB26773J

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