Issue 31, 2012

From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

Abstract

Polyhedral silsesquioxanes are considered valuable conjugation scaffolds. Nevertheless, only a few examples of silsesquioxane-assembled peptide oligomers have been reported to date. We developed a new bioorthogonal cube-octameric silsesquioxane (COSS) scaffold bearing eight aminooxy coupling sites allowing for the conjugation of diverse peptides via oxime ligation. We found that the coupling efficacy depends on the ligand in view of steric hindrance and electrostatic repulsion. For the first time scaffold-based conjugation of cystine-knot miniproteins having a backbone of about thirty amino acids was successfully accomplished without loss of bioactivity. Atomic force microscopy (AFM) provided further knowledge on the size of COSS verifying them as picoscaffolds growing upon bioconjugation to nano-dimension.

Graphical abstract: From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

Supplementary files

Article information

Article type
Paper
Submitted
13 Apr 2012
Accepted
11 Jun 2012
First published
13 Jun 2012

Org. Biomol. Chem., 2012,10, 6287-6293

From pico to nano: biofunctionalization of cube-octameric silsesquioxanes by peptides and miniproteins

S. Fabritz, S. Hörner, D. Könning, M. Empting, M. Reinwarth, C. Dietz, B. Glotzbach, H. Frauendorf, H. Kolmar and O. Avrutina, Org. Biomol. Chem., 2012, 10, 6287 DOI: 10.1039/C2OB25728A

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